Welcome to the Samara e-newsletter. The e-newsletter is published three times a year in March, June and September, starting September 2021. It aims to share up-to-date news and stories from across the Millennium Seed Bank Partnership (MSBP) as well as share information of relevance to the partnership.
The e-newsletter sits alongside the annual print version of Samara, published in December. More information and past issues of the print edition can be found on the dedicated Samara print edition webpage.
If you have any feedback or would like to get in touch with the Samara team, please email firstname.lastname@example.org .
Issue 7: September 2023
In this issue we hear about work in Corsica on two National Action Plans (plans nationaux d'actions - PNA). Ching-Yuan Hung, an MSB intern, shares the Kalanchoe project she worked on whilst on her internship and Vonona and Henintsoa talk us through the recent seed conservation techniques training course in Madagascar. Our species profiles this issue cover three threatened species from South Africa, and we interview Keith Manger who recently retired from the MSB after more than 25 years working for Kew.
Stories from the field and lab this issue explores the creation of two National Action Plans for a range of species from Corsica and about imaging techniques for the Kalanchoe genus.
National Action Plans (plans nationaux d'actions - PNA) are used in France as a way to focus on species for which conservation measures are needed.
For the Corsican flora, two PNAs are in progress. We presented in last December's Samara (issue 38), the first one, focussing on five Corsican Limonium species. The second concerns serpentinite vegetation in Corsica. For the two PNAs ex situ conservation actions include:
improving knowledge on viability, biology, phenology and germination conditions
developing genetically representative collections for each taxa in the seed bank.
These operations are financed by the French Ministry of Ecological Transition via the "Fonds vert".
What's new with the Limonium PNA? This year, Limonium seed lots harvested since 2019 were sorted into two groups. Some of the sorted seeds were placed in the National Botanical Conservatory (CBNC) seed bank, while others were subjected to various germination tests. When harvesting rare species, we don't exceed 25% of the total quantity of seeds in the population.
Germination tests, based on ENSCONET protocols, were carried out: 50 seeds (with the same initial appearance) were put on blotting paper soaked with distilled water. To find the optimum germination conditions and determine seed viability, the influence of two factors: light and temperature (15°C, 20°C, 25°C) were tested.
New tests will be carried out in 2024 to confirm the initial results obtained. These results will lead to a publication on Limonium germination conditions.
The CBNC is also working on the conservation of serpentinite species and vegetation in Corsica. The serpentinites (metamorphic rock rich in heavy metals) have a very high level of endemism, making a very special environment. In Corsica, there are various serpentinicolous outcrops, found in alpine areas, predominantly in Cap Corse and Castagniccia. This multi-taxa PNA will focus on an environment that needs to be preserved, but also needs to be better understood. Seven species, mostly or strictly serpentinicolous, are covered by this PNA: Alyssum robertianum, Biscutella rotgesii (protected by law), Scrophularia canina var. deschatresii, Elytrigia corsica (protected by law), Senecio serpentinicola, Echium montenielluense and Euphorbia pithyusa subsp. cupanii (protected by law). These seven species are endemic to Corsica, have a restricted range and are on the national and/or regional red lists. All of these taxa should be protected by law.
The development of this PNA started in 2023, and Action no. 9 of the PNA framework document focusses on seed bank development for Corsican serpentine taxa and the establishment of protocols for cultivation. It will also improve our knowledge of their germination through laboratory tests. In 2023, seed collection was initiated for all taxa at various locations. These harvests have already been sorted and banked. In 2024, germination tests will be carried out under different parameters. The results will be published.
Ex situ conservation contact: Caroline.Favier@oec.fr
Most of the species within the Kalanchoe genus are native to Madagascar, with some of them extending their distribution to regions such as Arabia and Asia (Vargas et al., 2022). However, human activities have led to the widespread presence of certain common species outside their original habitats, particularly those with ornamental value, resulting in some becoming invasive species (Herrando-Moraira et al., 2020; Vargas et al., 2022; Wang et al., 2016). Some Kalanchoe species are also known for their ornamental and medicinal uses in various countries (Akulova-Barlow, 2009).
Kalanchoe seeds are small and display brown to black colours, making it difficult to discern their texture with the naked eye. Despite their significance, research on Kalanchoe seed morphology has been limited, possibly due to their small size and ease of vegetative propagation. To address this research gap and develop a model for distinguishing seed morphology, a study was conducted using 34 Kalanchoe seed collections, respresenting 32 species in Kew’s Millennium Seed Bank at Wakehurst.
Image analysis was employed to quickly assess seed size, perimeter, solidity, and other relevant parameters, generating a comprehensive dataset for use in further investigations. Selected seeds were placed under a microscope, and pictures containing between 30 and 70 seeds were captured for ImageJ analysis (Figure 2). Additionally, higher magnification images were taken using focus-stacking technology, which combines pictures of the same object captured at different focal planes to create a sharp image with everything in focus. These focus-stacked images were linked to the online Data Warehouse of the Millennium Seed Bank Partnership (MSBP) (Figure 3).
The primary findings of the image analysis of bulk seed shapes and sizes will be published in a scientific journal to contribute to the existing knowledge base.
Overall, this research provides valuable insights into Kalanchoe seed morphology and establishes a framework for future studies on seed characteristics, which may have implications for conservation, horticulture, and other relevant fields.
Akulova-Barlow, Z. (2009) Kalanchoe. Cactus and Succulent Journal. 81: 268-276.
Herrando-Moraira, S., Vitales, D., Nualart, N., Gómez-Bellver, C., Ibáñez, N., Massó, S., Cachón-Ferrero, P., González-Gutiérrez, P.A., Guillot, D., Herrera, I., Shaw, D., Stinca, A., Wang, Z. & López-Pujol, J. (2020) Global distribution patterns and niche modelling of the invasive Kalanchoe x houghtonii (Crassulaceae).Scientific Reports.10.
Vargas, A., Herrera, I., Nualart, N., Guézou, A., Gómez-Bellver, C., Freire, E., Jaramillo Díz, P., López-Pujol, J. (2022) The Genus Kalanchoe (Crassulaceae) in Ecuador: from gardens to the wild. Plants. 11: 1746.
Wang, Z.-Q., Guillot, D., Ren, M.-X. & López-Pujol, J. (2016)Kalanchoe (Crassulaceae) as invasive aliens in China - new records, and actual and potential distribution. Nord. J. Bot.. 34: 349-354.
Across the MSBP article this month looks at how the Seed Conservation Techniques Course recently run in Madagascar went.
RBG Kew has been researching the Madagascar flora for over three decades. For the past 20 years, our seed conservation work through the Millennium Seed Bank Partnership (MSBP) programme has built a strong collaboration with Silo National des Graines Forestières (SNGF). This long-term collaboration has secured some 9,000 seed collections in the Madagascan national seed collection managed by SNGF. Since 2015, the work of the partnership between SNGF and the MSBP has been funded by the Garfield Weston Foundation, through the Global Tree Seed Bank (GTSB) programme. To date 2,500 seed collections have been made under this programme in Madagascar, representing at least 1,000 tree species conserved at SNGF and duplicated at the MSB in the UK.
From the 12th to 23rd June 2023, a training course on seed conservation techniques was held at SNGF. This initiative, part of the GTSB programme, aimed to provide training and technical advice to build the capacity of SNGF and the MSBP team in Madagascar to collect, store and manage high quality seed collections. The programme also aimed to strengthen the partnership between the MSBP teams from the UK and Madagascar and with the SNGF team. The training was delivered collaboratively by four members of the MSB team in the UK and three members of the MSBP team in Madagascar, who all brought different perspectives and areas of expertise to the training to ensure the programme was a success. The Head of the Production Department at SNGF also shared his expertise.
18 participants attended the course, including nine from the Kew Madagascar Conservation Centre (KMCC), seven from SNGF, one from Parc Botanique et Zoologique de Tsimbazaza (PBZT) and one from Centre ValBio at Parc Ranomafana. The mix of participants ensured an interesting breadth of experience was brought to, and shared throughout, the course.
The training covered a wide range of topics related to seed conservation. Participants learned about the MSB’s seed conservation standards, seed morphology, seed development and maturity, and building high-quality collections. They learned techniques for sampling genetic diversity, pre-collection assessment, germination testing, seed storage behaviour, germination and dormancy conditions, drying and the choice of seed storage containers.
To put their new knowledge into practice, the participants were divided into four groups. They carried out viability and germination tests, including cut tests, on eight different species. These practical exercises enabled participants to apply the techniques they had learned and to identify any gaps in their individual knowledge.
An essential part of the training was a field trip to a forest managed by the local community of Antavolobe, located in the eastern part of Madagascar, around 150 km from Antananarivo. Over five days, participants received in-depth training in methods for targeting seed populations, population assessments, cut tests, calculating seed availability, collecting seeds, the field data and preparing herbarium specimens. We also learnt about using a variety of equipment including field microscopes, hygrometers, pole pruners and a catapult. On the return journey the group stopped at a sacred site in the central highlands – Angavokely – to test their newly developed skills on seed collecting from non-tree species. The population assessment and collecting methods are the same regardless of the environment, but it was very interesting to see how the pre-collection assessment results differed for non-forest species, where the estimated total number of seeds was over two million, compared to just 300 or so for the tree species we had looked at over the previous days. We made two grass seed collections which have been duplicated to the MSB.
Back in Antananarivo, the participants had the opportunity to put their seed processing skills into practice. They learned techniques for cleaning, counting and preparing seeds for storage. Presentations were also given on managing seed data and monitoring seed viability. There was an opportunity for each participant to share their hopes for the future in terms of ex situ conservation of species through seed collection, and how they might work towards the realisation of these hopes through their own roles. In-depth discussions were held on collecting from rare and threatened species and from scarce populations, and on future restoration projects in Madagascar.
This training not only strengthened relationships among the various stakeholders (MSB , KMCC, SNGF, PBZT, and Centre Valbio) but also promised to make a positive and impactful contribution to our future work. It facilitated direct collaboration between the MSB Seed Collections team and their SNGF counterparts, allowing the exchange of expertise and experience for more effective long-term conservation of plants.
The training concluded with a quiz, followed by a group lunch, during which certificates were presented by the Director of SNGF, accompanied by the Conservation Partnership Co-ordinator for Africa from the MSB, and the head of KMCC’s Operations Team. This moment of celebration was an opportunity to recognise the efforts made by the participants and to underline the importance of their contribution to seed conservation in Madagascar.
The Garfield Weston Foundation have committed to funding the next phase of this programme, the Global Tree Seed Bank: Unlocked, which will run for three years from January 2024. This phase will see the MSBP team continue to partner with SNGF with a collection programme targeting endemic, rare and threatened species of trees and shrubs and other woody species that contribute to climate resilience, food security and livelihoods. The programme will also involve increasing seed supply for restoration projects in Madagascar, the development of improved facilities for germination testing and propagation at SNGF, the development of tools and skills for state-of-the-art species identification and work towards a deeper understanding of restoration ecology and species traits. Another training programme in Madagascar will be developed during year two of the GTSB: Unlocked.
At the end of August this year we said goodbye to Keith who retired after over 25 years at Kew.
What is your most memorable moment from your time working at the MSB?
This sounds really geeky, but I think it would be when we turned on the PVs (photovoltaic cells / solar panels) on the roof of the Millennium Seed Bank (MSB). I was heavily involved and was able to share my knowledge of the building. Kew’s Development team (previously Foundation) were critical in getting the financial case sorted. It was really significant with regards to reducing the building’s energy footprint. When the panels went live, that was an important moment for me. They have saved a massive amount of carbon (more than 300 tonnes) and currently deliver 7% of all our electricity at the MSB (about 550 MWhs). Getting the solar panels set up required working with the Estates team, Wakehurst staff and Foundation from within RBG Kew, and British Gas Solar (Centrica) outside of Kew.
More recently I assisted with getting PVs on the other half of the MSB roof, working with the Sustainability team.
What is your most memorable moment from an overseas trip?
What sort of memorable?!
Memorable for science, was the opening of the Kunming seed bank in China. Simon Linington and I had helped partners with the design of the very large seedbank facility. When we were invited out to see the finished seedbank it was amazing. Easily the biggest seed bank design I’ve been involved with.
If you could be a seed, which would it be?
A Mangrove seed – they’re really difficult to store (I always like to be awkward!), yet vitally important for conservation, and when someone cracks how to save them (store them in a seed bank) it will make a great paper.
What one piece of advice would you share to anyone working in seed conservation?
Don't under value the importance of what you’re doing! It’s not about financial value it’s about global value – no plants means no people or animals!
How did you get into Seed Bank design and management?
I was already well versed in lab design through my previous job, as I set up labs. Roger Smith and Simon Linington wanted to ensure their knowledge, which was huge, was imparted to someone else and that was me. I’d like to think I’ve done them justice. The MSB is a brilliant example of how to get it (mostly) right.
Where do you see the MSB in 20 years?
I see the building exactly where it is, as it was designed to last 500 years! But I would like to think its focus will remain on conservation and affiliated research. I would love to see the addition of a significant cryo-preservation and tissue culture facility so that we could work with taxa with recalcitrant seeds, that’s what I started working on in the 1980s.
Would you do anything differently if you could turn back the clock? And what advice would you have for colleagues going forwards?
If I could turn back the clock, I think I would like to have got on board a long-term external financial backer and developed a mandate that ensured that the building would primarily carry on doing what it was designed for, and that is both conservation and research.
Advice to colleagues going forward - I think it would be, don’t undervalue the partnership model and be flexible to change.
What on earth will you do with all the free time you have after you retire?
I’m not going to have any free time! I shall probably end up doing some voluntary work or something similar but probably in a different area. I intend to take it a bit easier and not be so rushed off my feet... and get a lot fitter!
What would the perfect seed bank look like and where would it be?
It would probably be similar to the MSB in that it would be designed to encourage people to work together as teams and have an efficient flow through the building. It would need to be adaptable to change, as would the people. It would also need to be properly maintained and most importantly it would need to link in with the larger seed bank community globally. The MSB is not a bad blueprint.
What is the biggest achievement from your work with Kew?
Getting ISO 14001 accreditation (environmental) has benefitted the MSB significantly and we are regarded as being highly proficient in this area. The other thing is helping our partners. I enjoy talking about the MSB and sharing my knowledge and I really enjoy training people and assisting our partners. This is really important to me, working with international partners and helping them help themselves. All this (the MSB) has been delivered through partnership. I’m still in contact with many of the people we have worked with.
Any last comments?!
You’ll achieve twice as much working together and don’t be afraid to ask for funding. If you’re passionate about what you’re doing, it’s half the battle won.
Brackenridgea zanguebarica by Naomi Mdayi (SANBI)
Brackenridgea zanguebarica is a threatened tree species that has been over harvested in the wild because of its bark and roots, which are used in traditional medicine. Due to its restricted occurrence and rarity, it has been listed as Critically Endangered (IUCN) and populations continue to decline in the wild. A partnership between MSBP South Africa, Mpendulo Gabayi (Tree horticulturist at Kirstenbosch National Botanical Garden), Thohoyandou National Botanical Garden, and the Missouri Botanical Garden in the United States was formed to conduct research on Brackenridgea zanguebarica. The project included monitoring the populations, seed collection and testing propagation techniques for this species. In addition, it is planned to augment and restore populations of this species in the wild. Ntsakisi Masia, joined Mpendulo Gabayi and Mpho Mathalauga at Brackenridge Nature Reserve, Limpopo, to conduct vegetative propagation (air layering) trials on Brackenridgea zanguebarica. Ntsakisi was tasked with monitoring the air layering experiment every three weeks over seven months at Brackenridge, Mutavhatsindi Nature Reserve in Thengwe, Limpopo Province. Ntsakisi, together with the Thohoyandou National Botanical Garden horticultural conservation worker, Rofhiwa Netshituni, and two horticultural interns, Thembuluwo Maduwa and Tshireletso Moloto, collected seeds of this CR species. The fruits were first covered in mesh bags to prevent birds and insects from feasting on them before they were ready to be harvested. Brackenridgea zanguebarica was successfully propagated through air layering - roots were observed in the dyna balls. Samples were taken to develop ex situ collections at Thohoyandou National Botanical Garden, Kirstenbosch National Botanical Garden and KwaZulu Natal National Botanical Garden.
Tulbaghia cominsii by Naomi Mdayi (SANBI)
Tulbaghia cominsii is listed as Critically Endangered on the Red List of South African Plants. This species is currently growing in one small patch along the N2 road in King Williams Town. This species grows on unprotected land and is at high risk of extinction since only one locality is known. The MSBP Seed Conservation Technician, Sibahle Gumede aimed to collect seeds for Tulbaghia cominsii conservation and for propagation in Kwelera National Botanical Gardens, to later restore to the wild. The project is still in the early phases of monitoring and targeting seeds. Sibahle Gumede collected the seeds of Tulbaghia cominsii between April and June 2021. On 13 August 2021 Sibahle Gumede and Nomama Mei started germination trials. Monitoring of the trials took place, and to date no seedlings have emerged. This could be due to a number of reasons, including the facilities used for germination. Sibahle Gumede is liaising with horticulturists from the Nelson Mandela Metropolitan University to assist with germination, as the university has better nursery facilities.
Syncolostemon incanus is a sparsely branched shrub, about 0.6 m tall. It occurs in the neighbourhood of the small town of Kaapsehoop in Mpumalanga. Syncolostemon incanus is assessed as Endangered (EN) in the wild. It is estimated that the population has been reduced by 50% in the past 100 years, based on the evidence that 76% of its habitat has been lost to forestry plantations and of the three known local locations, only one remains. Syncolostemon incanus leaves and flowers are used in potpourri mixture to provide a gentle natural scent in the home. The seeds and cuttings of this threatened species were collected in Kaapsehoop town in Mpumalanga Province in November 2021. Fergy Nkadimeng, the Seed Conservation Technician for the MSBP based in Pretoria National Botanical Garden in the Gauteng Province was joined by Tumelo Mofokeng and Bilaal Mansell-Jones, both from Pretoria National Botanical Garden on a trip to collect this species. Cuttings were collected to be propagated and grown in the Pretoria Garden as living collection. Unfortunately, these cuttings did not survive; propagation was attempted again using a different media and again, the roots did not develop. Another trial is planned for later in the season, when the seeds are ready for collection. Collections of Syncolostemon incanus represent a new species for at the MSBP
Opportunity for students and interns within the Millennium Seed Bank Partnership (MBSP) to get involved with the Samara e-newsletter
We’re looking to hear from you for the next Samara e-newsletter!
In this issue, Ching-Yuan Hung, a student intern who has recently finished a placement at the Millennium Seed Bank, has written about the project she undertook, focusing on seeds of the Kalanchoe genus. We would like to expand this, encouraging and supporting other students and interns across the Millennium Seed Bank Partnership to get involved with the Samara e-newsletter, including offering an opportunity to conduct an interview for the e-newsletter.
You could either interview someone at your own institution or we can pair you up with someone else at a different organisation within the MSBP. The editors of the Samara e-newsletter will be on hand to help throughout the process. The interview does not have to be done in English, we can add an English transcript to it during editing (both language versions will be published within the e-newsletter).
Please do share this with those who might be interested. If you would like to get involved or would like further details, please email us your name and organisation.
Equally, if you would be open to being interviewed by a student/ intern please let us know at email@example.com !
Issue 6: June 2023
In this issue we hear about field adventures in Indonesia collecting banana seeds and an invasive species in South Africa. In stories from across the MSBP we find out about a new exhibition on seed banking at the Canary Islands Botanical Gardens and plans for a partnership wide workshop on seed distribution. Rowan and Arthur (MSB interns) spoke with Dr Amelia Martyn Yensen, National Coordinator of the Australian Seed Bank Partnership, about her new role for "An interview with …". In the 2022 MSBP partnership questionnaire seed bank profiles were suggested as something you might like to see in the Samara E-newsletter, kicking off this new series we hear about the seed bank at UNAM, Mexico (available in Spanish and English). In News and Events we update on upcoming training courses at the MSB and how students and interns can get involved with the Samara e-newsletter.
Indonesia is part of the centre of origin and diversity of bananas, and therefore has a large number of both wild species and cultivars. Banana cultivars and landraces are mostly seedless. In Indonesia, they are extensively grown in home gardens, and very little as an estate crop (mostly Cavendish). Meanwhile, wild bananas are seeded and are commonly found in the wild forests. Conservation of wild bananas is essential to ensure the future of the modern crop. Conservation of crop wild relatives through seed collection is a suitable strategy to conserve germplasm, and conservation in gene banks is recommended as they are the reservoir of traits and genes required to face emerging abiotic and biotic stresses (Singh et al. 2021). Seed collection from wild populations will help maintain the high genetic diversity needed for further utilisation (Kallow et al. 2021). Wild bananas are under considerable threat due to habitat destruction, forest fragmentation and conversion, and other anthropogenic disturbances.
Seed collecting of wild bananas along with associated species has been conducted in two wild forests in Indonesia: Bromo Tengger Semeru National Park, East Java (2020) and Bogani Nani Wartabone National Park, North Sulawesi and Gorontalo (2022). Special thanks to the Arcadia Fund - a charitable fund of Lisbet Rausing and Peter Baldwin for providing expedition funds as part of the project ‘Threatened Biodiversity Hotspots Programme, Indonesia’, managed through the Millennium Seed Bank Partnership, Royal Botanic Gardens, Kew.
Bromo Tengger Semeru National Park is located in four regencies of East Java Province - Malang, Pasuruan, Probolinggo and Lumajang. It covers an area of 50,276 ha. This National Park has various tropical rainforest ecosystem types including lowland, sub-montane, montane and sub-alpine, with altitudes ranging from 750-3,676 m asl (Ariyanti et al. 2012). Seed collecting was conducted in October and November 2020.
The two wild banana species collected in Bromo Tengger Semeru National Park were both varieties of Musa acuminata - Musa acuminata var. nakaii Nasution and Musa acuminata var. rutilifes (Back.) Nasution. M. acuminata var. nakaii is easily distinguished by the purplish colour beneath the leaf and its hairy fruit. The leaves resemble var. zebrina, but differ in male bud tip and pericarp. Meanwhile, M. acuminata var. rutilifes is distinguished by the yellowish green colour of the petioles, midrib and leaves; and its small fruit with bottle-necked tip. These two varieties are widely distributed in Java, in open forest or along streams, at 50-1,500 m asl (Nasution, 1991; Hapsari, 2014). The associated species collected were mostly shrubs such as Acronychia trifoliolata, Eumachia montana, Ficus montana, Impatiens platypetala, Ricinus communis, Pollia hasskarlii, Phytolacca icosandra, Solanum betaceum, and Zapoteca tetragona.
Bogani Nani Wartabone National Park is the largest terrestrial conservation area in Sulawesi with an area of 282,089 ha. Administratively, this National Park is located in two provinces - North Sulawesi and Gorontalo. The biodiversity in Bogani Nani Wartabone National Park represents the richness and uniqueness of the flora and fauna of the Wallacea (Cannon et al. 2007). A seed collecting mission was conducted in September 2022.
Wild bananas collected from Bogani Nani Wartabone National Park were identified as Musa acuminata var. tomentosa (K.Sch.) Nasution and Musa acuminata ssp. banksii (F.Muell.) N.W. Simmonds. M. acuminata var. tomentosa is endemic to Sulawesi, distributed in lowland areas up to 600 m asl (Nasution, 1991). It is distinguished by its slightly angled to almost horizontal and spiral bunch- shape (all fruit is attached to a unique crown coiled around the stalk), lax to compact appearance, and blunt fruit tip. M. acuminata ssp. banksii is native to New Guinea, NE. Queensland, and Samoa. It has heavy bunches hanging downwards, a compact to very compact appearance, the fruit are curved towards the stalk and can be almost vertical, and the fruit are curved but are straight in the distal and pointed tips.
Associated species collected from Bogani Nani Wartabone National Park were trees, shrubs, herbaceous plants and orchids, including Begonia gambutensis, Begonia mendumae, Celosia argentea, Crotalaria juncea, Curculigo latifolia, Ficus ampelos, Hellenia speciosa, Homonoia riparia, Pollia thyrsiflora, Spathoglottis plicata, and Senna tora. B. mendumae is endemic to Sulawesi and has been proposed for IUCN category Vulnerable D2. It has unique asymmetric leaves, which are ovate with an acute tip, deep green in the centre and around the margins with darker mottling on the veins, a paler band running inside the margin; and tepals which are dusky pink combined with orange anthers and stigmas which make it very attractive (Hughes, 2006).
Seeds collected from both locations are now stored at the Seed Bank of Purwodadi Botanic Garden – BRIN, with duplication to the Seed Bank of Bogor Botanic Garden – BRIN, for further studies. Several seed studies are currently underway including periodic seed germination and viability testing, seed storage behaviour, seed morphometry characterisation, seed dormancy breaking and seed physiology and biochemistry. To date, seed studies from this project have resulted in two scientific articles published in national and international journals: Renjana et al. (2021) and Trimanto et al. (2022), respectively. One of the research topics has been presented at the Australasian Seed Science Conference 2021.
Ariyanti, E.E., Irawanto, R., Hapsari, L. & Mudiana, D. (2012) Distribution of Syzygium spp. (Klampok) in some areas of Bromo Tengger Semeru National Park. East Java. Proc. Soc. Indon. Biodiv. Intl. Conf.1: 135-142.
Cannon, C.H., Summers, M., Harting, R.J. & Kessler, P.J.A. (2007) Developing conservation priorities based on forest type, condition, and threats in a poorly known ecoregion: Sulawesi, Indonesia. Biotropica. 39(6): 747-759.
Hapsari, L. (2014) Wild Musa species collection of Purwodadi Botanic Garden: Inventory and its morpho-taxonomic review. J. Trop. Life Sci.. 4(1): 70-81.
Hughes, M. (2006) Four new species of Begonia (Begoniaceae) from Sulawesi. Edinburgh J. Bot.. 63(2&3): 191-199.
Kallow, S., Panis, B., Vu, D.T., Paofa, J., Mertens, A., Swennen, R. & Janssens, S.B. (2021) Maximising genetic representation in seed collections from populations of self and cross-pollinated banana wild relatives. BMC Plant. Biol.. 21: 415.
Nasution, R.E. (1991) A taxonomic study of the species Musa acuminata Colla with its intraspecific taxa in Indonesia. Memoirs of Tokyo University of Agriculture. 32: 1-122.
Renjana, E., Rahadiantoro, A., Ningrum, L.W., Lestari, D.A., Firdiana, E.R., Trimanto, Mas’udah S. & Hapsari. L. (2021) Inventory, morphological characterization, and seed viability test of Pacar Tere (Impatiens platypetala Lindl.) from the exploration in Bromo Tengger Semeru National Park. J. Pemuliaan Tan. Hut.. 15(1): 23-34.
Singh, S., Agrawal, A., Kumar, R., Thangjam, R. & John K.J. (2021) Seed storage behaviour of Musa balbisiana Colla, a wild progenitor of bananas and plantains – Implications for ex situ germplasm conservation. Sci. Hort.,. 280: 109926.
Trimanto, Renjana, E., Lestari, D.A., Firdiana, E.R., Mas’udah, S., Rahadiantoro, A., Ningrum, L.W. & Hapsari, L. (2022) Morphological characterisation and seed germination study of wild banana Musa acuminata var. flava (Ridl.) Nasution. J. Trop. Biodiv. Biotech.. 7(1): jtbb66645.
Cryptostegia madagascariensis is known as Madagascar rubber vine from the Apocynaceae family. The species is a perennial plant and native to North-Western Madagascar and invasive in Hawai'i and Australia. Cryptostegia madagascariensis is used as an ornamental plant due to its purple pinkish flowers. In other countries is it used as medicinal plants to cure ulcer and skin problems such as scabies. It spreads through seeds and flood water movement (McFadyen & Harvey, 1991; Starr, Kim & Lloyd, 2003). Cryptostegia madagascariensis is regarded as a 1b invasive species in South Africa and is a problem in three of the nine provinces of South Africa which are: Mpumalanga, Limpopo, and Northwest Province. No risk analysis has been conducted for the species in South Africa, but Cryptostegia madagascariensis tends to cover and climb trees. The species is poisonous to human beings and livestock. It may cause coughing and swelling of the nose when encountered as well as a blister to the eyelids (McFadyen & Harvey, 1991).
Cryptostegia madagascariensis has thick glossy and dark green leaves which are 2-11cm long and leathery. It has a pink to light purple tubular flower (30-40mm long) with five petals. The woody vine has milky sap, its stems are greyish-brown and have a whitish warty spots. It produces pods, 50-90mm long which contain numerous seeds. The shape of the seeds are oval-like (Acevedo-Rodríguez, 2005; McFadyen & Harvey, 1991). In South Africa Cryptostegia madagascariensis is treated mechanically and chemical is applied to reduce the spread of Cryptostegia madagascariensis.
Cryptostegia madagascariensis is naturalised and grows well in dry forests, roadsides, moist forests, rainforests, waterways, and waste areas. In communities, it grows along the fences as a climber. The public are encouraged to report sightings of this species as there is a need to protect local biodiversity. Reports of populations of Cryptostegia madagascariensis and other suspect species in South Africa are collected by the South African National Biodiversity Institute Directorate on Biodiversity Evidence (SANBI-DBE).
Acevedo-Rodríguez, P. (2005) Vines and climbing plants of Puerto Rico and the Virgin Islands. Contributions from the United States National Herbarium. 51: 483.
Foxcroft, L.C., Richardson, D.M. & Wilson, J.R.U. (2007) Ornamental plants as invasive aliens: problems and solutions in Kruger National Park, South Africa. Environmental Management. 41(1): 32-51.
MacFadyen, R.E. & Harvey, G.J. (1991) Rubber vine, Calystegia grandiflora, A major threat to natural ecosystems in Northern Australia. In: Eds. Centre, T.D., Doren, R.F., Hofstetter, R.L., Myers, R.L., & Whiteaker, L. Proceedings of the Symposium on Exotic Pest Plants, November 2-4, 1988, Miami, Florida. United States Department of the Interior, National Park Service, USA.
The Seed Bank of the “Viera y Clavijo” Botanical Garden recently inaugurated a new exhibition. The exhibition, carried out jointly with the Department of Environmental Education, aims to highlight the importance of seed conservation in germplasm banks as a way to conserve biodiversity.
It is organised into different panels that run through the hall of the germplasm bank. In the first introductory panel, visitors can see the importance that seeds have had and continue to have for humanity – seeds are considered as the basis of civilisation. A timeline indicates various milestones in the evolutionary history of seeds, from the appearance of the first seed plants approximately 350 million years ago, through the first cases of plant domestication by humanity on different parts of the planet (approximately 10,000 years ago), up to the appearance of germplasm banks.
The next panel tells us about how seeds are life carriers, how they are part of the biological cycle of plants, and how, with such a simple structure (seed coat, cotyledons and embryo), they can travel thousands of kilometres using the different forms of dispersal available to them.
Next, we find a panel that explains how biodiversity is processed in germplasm banks to preserve the viability of the seeds for as long as possible (Figure 1). In this panel, we see the different processes that take place, from sampling in the field to when the seeds are packaged and stored at -20°C, ensuring the traceability of the material in all phases of the process.
The relevance of germplasm banks for ex situ conservation as reservoirs of biodiversity is reflected in the following panel, where some of the most important seed banks, of the more than 350 that exist in 74 countries around the world, are located. In these germplasm banks, in addition to conserving wild species and crops, information on natural agricultural practices, natural medicinal uses, and culturally diverse species are also preserved.
The wealth of nature and biodiversity is reflected in the following panel, where images of seeds of endemic species from the Canary Islands under a stereoscopic microscope and an electronic microscope are displayed (Figure 2). In addition to showing the great diversity of seeds, reflected in their sizes, shapes, and colours, this section reveals the impressive world seeds hide inside. This panel conveys to those who visit the exhibition the idea that biodiversity is found at different scales from species and, ecosystems, to genetic and morphological diversity.
Next to the biodiversity section, we find a panel on threats to biodiversity: degrading ecosystems. Here are reflected the main threats currently to species: introduced species, habitat alteration and destruction, and over-exploitation. In this area, human beings see themselves as one of the main threats to biodiversity.
The purpose of this exhibition is to introduce visitors to the world of seeds, their importance in the biological cycle of plants, their biology, conservation, and diversity which travel through time and space.
The aim of the workshop will be to empower everyone within the MSBP to undertake the distribution of seeds and to grow demand for a wider range of species. As part of the workshop, we hope to bring together many case studies of different approaches to seed distribution from across the partnership, including different tools available to help. In addition, we will facilitate discussion through break-out sessions focussed on the challenges we all face in seed distribution. We recognise that aims and scales of seed distribution can vary drastically from small seed number requests primarily for standard research, through to larger scale to support restoration requiring growing out of collections. Our hope is that through the workshop, new working relationships can be developed to support each other with seed distribution. Overall, we will bring together as much of the information from the workshop as possible into a technical information sheet.
If this workshop goes well, hopefully workshops will be something we can run more frequently across the partnership. Timing the event will be one of the more challenging aspects and we will consider a variety of different options to make it as accessible to all as possible.
Further details will be released in the coming months. In the mean time, if you have any ideas of suggestions for the workshop please contact your MSB country coordinator or email firstname.lastname@example.org .
Interview by MSB Kew interns Rowan Black and Arthur Butler
Dr Amelia Martyn Yenson is the new national coordinator of the Australian Seed Bank Partnership (ASBP). The partnership is an alliance of botanic gardens, seed banks, non-government organisations and state conservation agencies. The aim is for national collaboration to advance conservation and seed banking to look after Australia’s natural heritage.
Having recently started the role, what are you most looking forward to?
This is my third week on the job, so there is lots I'm looking forward to! I love talking to other plant lovers and working with botanic gardens is such a privilege. I've been involved in the Australian seed banking effort and the Millennium Seed Bank project since 2004. So, I've developed networks across the partnership, but I'm looking forward to meeting new people with different roles in ASBP. I'm interested in what we can do collectively … what resources and training opportunities we can advocate for and learning about what people do daily in their seed banks, their challenges, and successes, and how the Partnership can best support them.
With the partnership's success so far, could you see the expansion of the collaboration on a regional level (Australasia's Oceania) rather than a purely country level?
We've built a strong network in Australia and have long been documenting our understanding of seed biology, germination, dormancy, and storage behaviour. There is plenty of interest across Oceania in better collaboration. In my previous role working for the Australian Network for Plant Conservation, I was involved in discussions at the Australasian Seed Science Conference and the 7th Global Botanic Gardens Congress. We supported ASBP as they began to explore what a broader network could look like, and with Aisyah's support from the MSBP, it's an area we’d like to continue to progress. We must understand what partners want from collaboration and the challenges they face due to limited time and resources, with many already stretched and overwhelmed by the day-to-day demands of conservation.
With the support of ASBP, we organized a Fenner Conference for the Environment funded by the Australian Academy of Science, within the Australasian Seed Science Conference in 2021. With their support, we made it a free event allowing regional participation. There was great interest and collaborative knowledge-sharing by researchers and national and international collaborators. Informal networking is common at the individual level for personal research networks, but links could be formalized to broaden the partnership and its impact.
With a rich history and knowledge of Australia's wildlife and plants, how are indigenous people and their knowledge being integrated into the work you do across the partnerships?
We must acknowledge First Nations peoples and their extensive knowledge, having lived and cared for Australia's Country for tens of thousands of years. This means the timeframe we have been practicing conservation and the duration that we aim to store seed occupies only a fraction of this timeline of care. Our partners have been fortunate to develop collaborations with First Nations peoples through a range of projects over the last few years.
Within Australia's First Nations cultures, there are hundreds of languages and peoples who have unique connections to their local environment. While we aim to work Australia-wide, starting at a local level with our partners already engaging with First Nations communities is essential. We aim to understand what they would want from a partnership and where they would like support. There is still a lot to learn and it's essential to respect the intellectual property developed in each collaboration.
The recent Florabank guidelines include a chapter about indigenous seed use and respectful working methods through an approach known as "Right Way collaboration". The chapter was led by Stephen van Leeuwen, a respected First Nations person; thus, it's a resource that we can confidently use and share. The Commonwealth Scientific and Industrial Research Organization (CSIRO) and other Australian organisations have produced informative documents on engaging respectfully and "Right-Way.” It's a continuous learning journey for us, but the best start is to nurture those individual connections and help our partners who work on Country to do what they already do with increased and ongoing support.
Was there a specific moment where you were like, "Yep", I know this is what I want to do for my career?
I was always interested in plants. As a child, my family would take me to the Royal National Park, an old, well-established National Park close to Sydney, where we would go on picnics and bush walks. When I finished high school, I remember sitting in the school hall with a manual listing all the university courses, and for the first time, the University of Sydney offered a Bachelor of Horticultural Science. It listed all the things we would learn, which sounded terrific. It also said we might have the opportunity to go to Kew, and I thought, oh my gosh, this is perfect; this is the course I'm applying for!
Is there any advice you can give a young person looking to get into seed science or plant conservation?
It's about following your passion and doing what you love. I wanted to work with Australian plants because they're beautiful, and I have since realized through my experience how important they are to ecosystem function.
Plants are fascinating, and sharing this excitement with other people is essential. This can be tricky, as I've noticed with my kids that sometimes the first thing they hear about the environment is ‘doom and gloom’. Thus, they are worried or scared before they even have a chance to experience the incredible diversity, the scents, the sights, the colours, and how wonderful nature is. We must keep that excitement alive while also making space for those hard conversations in our work together. There is still much to learn, so my colleagues and I will never be bored!
How is climate change challenging conservation in Australia outside of some of the more well-known impacts like increased incidence and ferocity of bushfires?
Climate change exacerbates all the processes that threaten biodiversity and plants, like land use change, changing fire regimes, pests, and diseases. The combined effect of these threats is causing the most damage. Within the partnership, we have projects focusing on the areas most at risk from this rapid change.
For instance, our partners study tropical mountain top plants and this is often the first opportunity to collect plant material or study the seed biology of these species. Their seed biology can be challenging, and we cannot always collect seeds, so instead they may collect material to establish living collections. Rainforests including tropical mountain tops and alpine areas have a high potential for climate change impacts, and the species in those habitats are at risk. This area of research is rapidly evolving, with numerous experts studying its impacts in and around Australia.
How often do you come across exceptional species in your projects? What problems are they causing?
This is a real area of interest for me and my colleagues. The focus of the Fenner Conference for the Environment was exceptional species. On the second day, we held an expert workshop, where based on Valerie Pence's framework (Pence et al. 2022 Biological Conservation), we started making a running list of exceptional species in Australia. The framework is a useful tool to help determine the best form of ex situ conservation for a non-orthodox species. Before starting my new job, my colleagues and I submitted a paper that applies the exceptional species framework in Australia, creating a workflow that will hopefully lead to improvements in ex situ collections and prioritize research.
To what extent do commercial partnerships play a role within the network, say with regeneration and restoration initiatives tied with the mining industry?
Government agencies often fund conservation initiatives, on a national basis and at State and Territory levels. Due to the scale of the biodiversity crisis we face, we also rely on non-government agencies, commercial partners, and philanthropic initiatives to fund projects.
In terms of mining companies, they are legally responsible for restoration. Some partners have a level of collaboration with mining companies who invest in best-practice, but our conservation seed banks are generally not equipped to practice large-scale restoration because collections are mostly small. These projects require scaling up from small packets to tons of seed. The mining industry does try to encourage natural seed regeneration from the soil seed bank to reduce reliance on tubestock planting and direct seeding.
How do you see technology changing the way we bank seed? Will robots, let's say, be running our seed banks in 10 to 20 years?
People will always have a role because seeds are so diverse that you will always need people to feed the machines the correct information and do the delicate, hands-on work! I'm interested in how new technologies are used in crop gene banks to facilitate their testing and regeneration at scale.
I'm keen to learn more about how technology can be used for broad-scale restoration. In Australia, a company called ‘AirSeed’ uses drones for restoration activities. Some scientists are looking at the best evidence-based ways to use drones, for example, with seed collection, they have the potential to help collect from inaccessible populations.
Our final question, possibly the most important, which seed could you throw the furthest?
I won't be the one to throw it; I will let my 13-year-old cricket-mad son throw it! You don't want something that's wind-dispersed as it would fly away, and you don't want something massive like Coco-de-Mer (Lodoicea maldivica) because it's too big. I think something that could fit in my hand like a Black Bean tree seed (Castanospermum australe) would be a nice size and shape to throw.
Isela Rodríguez Arévalo, Aramando Ponce Vargas (FESI-UNAM) & Michael Way (RBG Kew)
In the seed bank collections we maintain viable populations of wild and native Mexican species, especially those at risk. The BS is at the FESI campus in Tlalnepantla in the northwest of Mexico City and is linked to the main University City in the south of the city, and with other UNAM centres around the country.
Over time, the BS has conserved native and wild flora by region (Tehuacán-Cuicatlán Valley, Sierra Queretana, Sonoran Desert, etc.). For the past 10 years, the focus of the collections has been on useful trees in Mexico, particularly in the state of Veracruz, as it is a "hotspot" for the diversity of Mexican trees.
The management of the collections in the BS follow international quality standards stipulated by ISTA and procedures are evaluated by the Millennium Seed Bank standards team.
Equipment is expanded and updated with each project, currently the BS has a dry room (modified from a large growth chamber), seven freezers for long-term storage and two germination incubators for viability tests.
In addition to FESI research and management staff, there are project funded technician positions and currently 11 students who help with the manual processing of collections. There is also a small herbarium (IZTA) and a large greenhouse which has been used for research and propagation experiments.
It is estimated that in Mexico there are a total of 23,314 species of vascular plants, and after 20 years of continuous work, the BS collection totals 2,756 species, that is, 11.82% of the national vascular flora. The total number of accessions conserved is 4,980 and they belong to 180 botanical families and 985 genera, which have been collected from 26 of the 32 states in the country.
A general analysis of the conservation status of the collections showed that 864 species, that is, 31.35%, are endemic to Mexico. In accordance with official Mexican standards, this includes species under some category of risk, from which 31 species are subject to special protection, 28 species are classified as threatened and 15 are in danger of extinction. Thus, 2.7% of the conserved species are at some type of risk.
The families in danger of extinction with the largest number of species and protected in the BS are: Fabaceae, Fouquieriaceae, Pinaceae and Zamiaceae, all families that are widely exploited to take advantage of their wood or for use as ornamental species.
The Asteraceae family is the best represented in the BS, however, only two of its species are under some category of risk, these are: Hazardia orcuttii and Zinnia violaea.
One of the best represented genera in the BS is Agave, and particularly one of its species, A. peacockii. This species is subject to special protection (through NOM-059), a situation that is related to its use for the production of one of the most famous alcoholic beverages in the country, Mezcal. This situation is aggravated as the species is endemic to Mexico.
Undoubtedly, the conservation efforts of the BS are vital and fundamental to safeguard the native and wild biodiversity of this megadiverse country. Mexico has the possibility of safeguarding its plant genetic resources in the BS, and of participating in the great Millennium Seed Bank Partnership. Collaborations like these must continue and strengthen to minimize the effects of human intervention in the disappearance of native plant species around the world.
Con el objeto de reducir las posibilidades de extinción de especies con problemas de conservación, surgió en 2002 el Banco de Semillas Facultad de Estudios Superiores Iztacala - Universidad Nacional Autónoma de México FESI-UNAM (BS) en México, con el apoyo y financiamiento del Millenium Seed Bank del real Jardín Botánico de Kew. En esta colección mantenemos viables poblaciones de especies silvestres y nativas, especialmente las que viven bajo algún tipo de amenaza. El BS se encuentra ubicado en la Facultad de Estudios Superiores, campus Iztacala en Tlalnepantla, al NW de la Ciudad de México.
A lo largo del tiempo, el BS ha conservado flora nativa y silvestre de manera regional (Valle de Tehuacán-Cuicatlán, Sierra Queretana, Desierto Sonorense, etc.). Desde hace 10 años, el enfoque de las colectas se ha centrado en árboles útiles de México, particularmente en el estado de Veracruz, por tratarse de un “hotspot” para la diversidad de áboles mexicanos.
El manejo de las colecciones en el BS de apega a los estándares internacionales de calidad dictados por la ISTA y evaluados periódicamente por el equipo del Millennium Seed Bank.
El equipo con el que cuenta el BS, se mejora y se incrementa con el desarrollo de cada proyecto, actualmente el BS cuenta con un cuarto de secado con temperatura y humedad controladas, 7 congeladores para el almacenamiento de las colecciones y 2 incubadoras para los análisis de viabilidad.
El equipo de investigación y el cuerpo técnico está compuesto por 11 estudiantes que llevan a cabo el procesamiento de las colecciones, 1 colector botánico y taxónomo, un curador especializado en taxonomía y etnobotánica y conservación y un responsable del proyecto especialista en recursos naturales, taxonomía y conservación.
Se calcula que en el país hay un total de 23,314 especies de plantas vasculares, actualmente y luego de 20 años de trabajo continuo, la colección del BS está formada por un total de 2,756 especies, es decir, el 11.82% de la flora vascular nacional. El total de nuestras accesiones bajo resguardo es de 4,980 y pertenecen a 180 familias botánicas y 985 géneros, mismas que se han recolectado en 26 de los 32 estados del país.
Un análisis general del estado de conservación que presentan nuestras colecciones, arrojó como resultado que 864 especies, es decir el 31.35%, son especies endémicas de México. De acuerdo con el listado de la Norma Oficial Mexicana 059 (2010), la cual incluye especies bajo alguna categoría de riesgo, resguardamos 31 especies sujetas a protección especial, 28 especies están catalogadas bajo amenaza y 15 se encuentran en peligro de extinción. De manera que el 2.7% de las especies resguardadas, se encuentra bajo algún tipo de riesgo.
Las familias en peligro de extinción con mayor número de especies y resguardadas en el BS son: Fabaceae, Fouquieriaceae, Pinaceae y Zamiaceae, todas, familias que se explotan ampliamente para aprovechar su madera o utilizarlas como especies ornamentales.
La familia Asteraceae es la mejor representada en el BS, sin embargo, solo 2 de sus especies, se encuentran bajo alguna categoría de riesgo, según la NOM 059, éstas son: Hazardia orcuttii y Zinnia violaea, resguardadas en el BS.
Uno de los géneros mejor representados en el BS es, sin duda, el género Agave, y, particularmente, una de sus especies, A. peacockii esta especie se encuentra sujeta a protección especial, de acuerdo con la NOM-059, situación que seguramente está relacionada con su uso para la producción de una de las bebidas alcohólicas más famosas del país, el mezcal. Esta situación se agrava, cuando observamos que se trata de una especie endémica de México.
Sin duda, el esfuerzo de conservación del BS es necesario y fundamental para resguardar la biodiversidad nativa y silvestre de un país megadiverso. México, tiene la posibilidad de resguardar sus recursos genéticos vegetales en el BS, pero también, de participar en el gran proyecto de conservación del Millennium Seed Bank. Proyectos como estos deben seguir existiendo para minimizar los efectos de la intervención humana en la desaparición de especies vegetales nativas en el mundo.
Upcoming training opportunities at the MSB
We are currently looking for participants for two courses taking place later this year.
In September (18th to 29th) we will offer our annual Seed Conservation Techniques course which will be fully online this year. Please see Seed Conservation Techniques webpage for further details and apply by the end of June.
For the first time we are also organising a course in plant cryopreservation from the 30th October to the 9th November, which will take place at the Millennium Seed Bank and include practical sessions on methods like cryopreservation of embryos and shoot tips. For further information please contact MSB Training: MSBTraining@kew.org .
Opportunity for students and interns within the Millennium Seed Bank Partnership (MBSP) to get involved with the Samara e-newsletter
We’re looking to hear from you for the next Samara e-newsletter!
You could either interview someone at your own institution or we can pair you up with someone else at a different organisation within the MSBP. The editors of the Samara e-newsletter will be on hand to help throughout the process. The interview does not have to be done in English, we can add an English transcript to it during editing (both language versions will be published within the e-newsletter).
Please do share this with those who might be interested. If you would like to get involved or would like further details, please email us your name and organisation.
Equally, if you would be open to being interviewed by a student/ intern please let us know at email@example.com !
Recent publications from across the MSBP:
Batista, G.S., Ferraz, M.V., Mazzini-Guedes, R.B., Pritchard, H., Marks, T.R., & Pivetta, K.F.L. (2023) Germination response of palm seeds on a two-way thermogradient plate. Revista Ceres. 70(2): 49-57. DOI: https://doi.org/10.1590/0034-737x202370020006
Chen, H., Visscher, A.M., Ai, Q., Yang, L. & Pritchard, H.W. (2023) Intra-specific variation in desiccation tolerance of Citrus sinensis 'bingtangcheng' (L.) seeds under different environmental conditions in China. International Journal of Molecular Sciences. 24(8): 7393. DOI: https://doi.org/10.3390/ijms24087393
Dayrell, R.L.C., Ott, T., Horrocks, T. & Poschlod, P. (2023) Automated extraction of seed morphological traits from images. Methods in Ecology and Evolution. DOI: https://doi.org/10.1111/2041-210X.14127
Liu, U., Gianella, M., Dávila Aranda, P., Diazgranados, M., Flores Ortíz, C.M., Lira-Saade, R., Bacci, S., Mattana, E., Milliken, W., Mitrovits, O., Pritchard, H.W., Rodríguez-Arévalo, I., Way, M., Williams, C. & Ulian, T. (2023) Conserving useful plants for a sustainable future: species coverage, spatial distribution, and conservation status within the Millennium Seed Bank collection. Biodiversity and Conservation. DOI: https://doi.org/10.1007/s10531-023-02631-w
Mattana, E., Chapman, T., Miles, S., Ulian, T. & Carta, A. (2023) Regeneration from seeds in a temperate native flora: A climate-smart and natural-capital-driven germination risk modelling approach. Plants, People, Planet. DOI: https://doi.org/10.1002/ppp3.10378
Issue 5: March 2023
In this issue we hear about field and lab adventures from China, South Africa and the UK. In 'Across the MSBP' Arthur, Lola and Rowan, current MSB interns, tell us about the projects they're working on, and we hear updates on the recent Exceptional Species training course and progress on actions from the 2022 MSBP partnership questionnaire. This quarter's species profiles come from South Africa - Leucadendron and Apodytes abbottii. Michael Way shares his experiences of working at the MSB in 'An interview with' and in the news and events section we share updates on the Seed Information Database and a new seed exhibition at Jardín Botánico Canario "Viera y Clavijo".
Stories from the field and lab this issue explore the germination of a rediscovered aquatic plant from China, the impact of an invasive species from South Africa and the relationship between seed collecting and roads.
Hydrocera triflora (L.) Wight & Arnott is a perennial, hydrophytic plant with pink or yellowish flowers belonging to the Balsaminaceae (touch-me-not family). Its conservation status was evaluated as Regionally Extinct (RE), according to the Red List of Biodiversity in China (2013), until researchers from Kunming Institute of Botany rediscovered wild populations in Hainan Province in 2014.
Most members of the touch-me-not family produce touch-sensitive fruit, with explosively dehiscent capsules. The fruit of monotypic H. triflora is a drupe with pyrenes, but there is little known about the seed biology of this species. Understanding the seed germination and storage behaviour is important to develop a conservation strategy. Our recent studies discovered that fresh seeds of H. triflora had non-deep physiological dormancy, and germination percentages up to 96.8 ± 1.37% at alternated temperature of 35/25°C after warm stratification for 42 days at 20°C on 1% water agar were achieved. Dry seeds keep well with a germination rate at 96.8 ± 1.3% when seed moisture content (MC) was reduced to 4.36 ± 0.02% (fresh weight basis). Germination percentage remained at 94.8 ± 1.6% after 1 year stored at -20°C.
Seeds of H. triflora survive after drying and can be stored under conventional seed storage conditions for long-term preservation. The germination protocol and enhanced seed knowledge of H. triflora may boost conservation and restoration of this species.
Chinese Academy of Sciences and Ministry of Environmental Protection of the People's Republic of China (2013)Red List of Biodiversity in China-Higher Plants.
A new invasive species (Salvia coccinea) has been detected in the cemetery at the Lowveld National Botanical Garden (LNBG), South Africa. The cemetery belongs to the City of Mbombela and is situated on the outskirts of the Garden near the dumping site. The area infested by Salvia coccinea in the LNBG is estimated to be 0.08 hectares (Fig. 1).
Salvia coccinea is a beautiful plant, but a potentially nasty emerging invasive plant. In the LNBG it was first detected next to the gate to the dumping site in 2017, with Garden staff unaware that it would become a serious invader. Salvia coccinea is not regulated under South African law.
Salvia coccinea (also known as Scarlet Sage) is a herbaceous plant,belonging to the Lamiaceae family. It was first introduced to the Garden as an ornamental, and a traditional medicinal plant (Li et al., 2013). It usually grows up to 1.5 m tall. The stems are covered with short hairs, and long spreading hairs during the juvenile stage. It has dark green leaves, and when flowering it produces bright red flowers, with small upper lobes (Fig. 2).
Salvia coccinea is spread through seeds. These seeds can be dispersed naturally by wind or water, while people often spread it in dumped garden waste. Salvia coccinea grows well in shady areas.
Distribution and habitat
Salvia coccinea originates in the Southern United States, Mexico, Central and South America. According to the Southern African Plant Invaders Atlas (SAPIA), Salvia coccinea has invaded six South African provinces.
The plant survives well in disturbed sites such as cemeteries, open wasteland, roadsides, open urban areas, gardens, plantations, open woodland, and riparian vegetation. It grows well in warmer temperate regions. Not only the LNBG bears the brunt of Scarlet Sage, but also some other parts of Mpumalanga and Limpopo are affected.
Salvia coccinea seems to thrive during periods of high rainfall and flooding. This theory proved to be correct in 2020, after a massive infestation was discovered shortly after heavy rainfall and flooding was experienced during the summer rainfall season. Subsequently this plant has become a common sight in Mpumalanga and Limpopo.
Environmental impact and other potential impacts
Salvia coccinea is regarded as an environmental weed in Queensland (Australia), where it forms dense stands, dislocating native plant and animal species (CABI, 2021; Hindmarsh, 1937; Jäger and van Staden, 2000).
Another potential impact of Salvia coccinea include being poisonous to domestic animals, such as cattle. The young plants are said to be more toxic to cattle. After consumption, weakened and awkward movements have been observed.
Management and research
After Salvia coccinea was classified as a suspected potential invader, a detailed survey was conducted across the LNBG to check for more populations and discover the extent of the infestation.
The first survey was done in November 2021 at the previously mentioned graveyard site. The following organisations participated in the survey: South African National Biodiversity Institute - Directorate on Biodiversity Evidence (SANBI–DBE); Groen Sebenza Interns; and Department of Fisheries Forestry Environment (DFFE). The distribution was significantly wider than initially thought having spread from the cemetery (Fig. 3).
The next survey was conducted by the SANBI Curator and SANBI-DBE staff. It was conducted in the southern part of the Garden, on the Garden Estate site bordering the University of Mpumalanga. During this survey (conducted in December 2021), only one plant was spotted along the fence on the Estate site of the Lowveld National Botanical Garden.
SANBI-DBE is planning to remove populations of Salvia coccinea detected within the LNBG before it spreads to other parts of the Garden. The Garden plans to use the Eco–monitors team.
Currently, there is no herbicide registered to control the spread of Salvia coccinea in the Garden. In the meantime, the SANBI–DBE team will be using an uprooting method to control the outbreak. In addition, the team will be on the lookout for populations within the Garden and will gather data for future analysis. Data collected will be stored in accordance with SANBI-DBE records and data storage methods.
For several years, SANBI has been actively working towards controlling and preventing the spread of alien invaders in the Garden. During the last financial year, through support of the PEPSI budget, over 30 local people were employed by SANBI to clear alien invasive plants in the Garden.
Further surveys will be conducted to determine the distribution of Salvia coccinea outside the LNBG, across Mpumalanga, and other provinces in South Africa. In addition, a risk analysis will be conducted to regulate the species.
Hindmarsh, W.L. (1937)Salvia coccinea. A garden escapre, poisonous to stock. N.S.Wales Res. Rept.7: 118.
Jäger, A.K. & van Staden, J. (2000)Salvia in southern Africa. In S.E. Kintzios (Ed.) Sage: The genus Salvia. Harwood academic publishers, Amsterdam.
Li, M., Li, .Q., Zhang, C., Zhang, N., Cui, Z., Huang, L. & Xiao, P. (2013) An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China. Acta Pharm. Sin. B.3: 273-280.
Nagel, K.B., Gupta, A. & Asrani, R.K. (2014)Salvia coccinea poisoning among migratory Gaddi goats: Evidence from mid-hills of Himachai Pradesh (India). Indian J. Anim. Sci.84: 37-38.
Ex situ seed conservation relies on access to seed sources, but how much of it is driven by proximity to roads and paths? To investigate this question, I’ve used a subsample of georeferenced seed collections available from the MSBP’s Data Warehouse (n > 42,000) and extracted from OpenStreetMap the shortest distance to a road and the type of road.
At a global scale, results show that 90% of all seed collections are georeferenced within 800 m of a road (approximately a ten-minute walk). However, almost half of them (49%) returned with distance values less than 10 m away from a road (Fig. 1), highlighting the role of roads in which plants seeds are collected from. The further away a collection point is from a road, the more likely it is the distance result is biased by a lack of information on roads in that area. OpenStreetMap is an ongoing mapping project, with remote areas still lacking precise information. Some notable exceptions are found in Australia, where some collection points are kilometres away from any visible road (Fig. 2). Another factor affecting the results are collections geotagged in batches (several collections sharing the same coordinates, see Fig. 1). Additionally, collections might have been made back when roads nearby were not around, but results have been obtained using the latest information on road maps.
At a regional level, patterns in distance from the nearest road to collection location is like those at a global scale (Fig. 3), but road types differ between regions (Table 1). For example, Europe shows less collections obtained near primary roads although has a higher proportion of collections obtained from tracks and paths. Europe also has the lowest number of unclassified roads, but this is probably due to OpenStreetMap road maps information being more accurate than in other regions.
Table 1: Percentage of road types for nearest road to a collection by region. * = not necessarily available to motorised vehicles. See references and resources section for OpenStreetMap Wiki (2023).
Primary Roads (%)
Secondary Roads (%)
Tertiary Roads (%)
Trunk Roads (%)
Residential Roads (%)
Service Roads (%)
Based on these results, it seems like ex situ collectors favour conservation of seeds from accessible plants near roads, meaning that the further away plant resources are from a road, the less likely these genetic resources will be represented in a collection. While more representation from less accessible plants would improve the genetic variability of current collections it could also be argued that “inaccessible” plants are less at risk from the direct and indirect anthropogenic impacts caused by roads.
Many thanks to Aisyah Faruk, Matthew Jeffery and several principal collectors for their help and ideas.
In 'Across the MSBP' this issue, three students currently undertaking a year-long internship at the MSB between their second and third years at university tell us about the projects they're working on, Hanna Oldfield, training coordinator at the MSB, gives an overview of the recent exceptional species training course, and we also provide an update on the progress of our actions arising from the 2022 MSBP partner questionnaire.
This year at the MSB, we have three 12-month interns working on various projects. They are all between their second and third years of their university degrees and have taken some time to write a little about the projects they're working on.
Using seed traits to mitigate invasiveness risk of American prairie species at Wakehurst by Arthur Butler, Michael Way (RBG Kew) & Rafia Sultana Hogg (Capel Manor College)
An occupational hazard of the management of botanic gardens comes when introducing multiple species to an unfamiliar ecosystem. While some species may struggle to gain a foothold, others have the potential to thrive, sometimes even outcompeting local flora. With the number of invasive species up 70% since 1970 (IPBES 2019), finding ways to mitigate risks when creating a novel plant community is of paramount importance. In botanic gardens, where plants often have significant conservation value, even more so. We used seed traits to assess and help managers minimise these risks.
In March 2022, a project to mitigate the effects of introducing wild origin species from the USA to a UK botanic garden began. The investigation centred around the six-acre American prairie, created beside the formal gardens at Wakehurst. The prairie hosts 110 species, planted in 2020 using wild sourced seed, creating a novel habitat within the botanic garden. Due to their success in similar climates across Europe the following six perennials were chosen for study: Coreopsis lanceolata (CL), Eryngium yuccifolium, Liatris aspera, Solidago rigida (SR), Veronicastrum virginicum and Zizia aurea. During the project it was discovered that 10% of seed present in the prairie’s soil seed bank belonged to a seventh species: Rudbeckia hirta, which has since been added to the focal species list.
The ‘American Prairie Risk Ranking’ (APRR) was created to appraise invasiveness potential. Through spring and summer 2022 physical characteristics were recorded (e.g., plant height and seed production are key contributors to potential invasiveness). Seed trait data was collected throughout autumn, with a focus on seed dispersal and viability. These data alerted us to the invasiveness potential of SR and CL, which both displayed high numbers of viable seeds. CL was abundant in the American Prairie whilst SR could disperse seeds over long distances.
Each passing year presents an opportunity for further data collection and analysis, which will help refine and strengthen the APRR framework.
Climate smart seed selection by Lola Andrews (she/her)
Biodiversity is fundamental to a living planet, however our current way of life does not support biodiversity, we are destroying it at an alarming rate. I am sure that is not new to any of you and if you are anything like me you all have pondered various (wild and wacky) ways of tackling this issue.
One of my roles on this project is to carry out seed germination experiments. UK native grassland species have been selected based on their vulnerability and distribution within the UK. To model their germination response to temperature, the seeds are incubated at different temperatures, ranging from 0-40°C. Two of the species studied are Common Knapweed, Centaurea nigra L., and Yellow rattle, Rhinanthus minor L. (Fig. 1). By monitoring their germination kinetics across this temperature gradient, we can assess which species or populations are more sensitive or resilient to climate change. This involves spending a lot of time in the lab, peering over thousands of seeds on agar and carefully checking each one for a protruding radicle. Luckily, this is something I really enjoy. Sometimes it feels like a game of hide and seek, as in some species the radicle gets tucked under the seed or into the agar, making it inconspicuous- it is rewarding to find those cheeky germinated seeds.
By testing a number of different species, and populations within species considered to be at risk, we can begin to build a picture of what our future environment/landscape might look like. With this information climate smart seeds can be selected, either from resilient species or resilient populations, to be used in restoration projects nationwide. This approach will support more targeted and resilient restoration activities.
Make no exception for the exceptional by Rowan Black
Exceptional describes a plant species that cannot be seed banked under standard conditions due to its inability to complete one, or more, of the four steps in the orthodox seed banking process (collection, processing, storage & recovery). The term was formally defined in 2022 by Valerie Pence and colleagues in two companion papers (Pence et al. 2022a & b). Alongside the papers, 23,530 plant species were used to form a list which assigned 775 species as exceptional. However, 17,603 species had insufficient data to be classified (Pence et al 2022b). Comparing the exceptional species list from Pence et al. (2022b) with Millennium Seed Bank (MSB) and Millennium Seed Bank Partnership (MSBP) base lists, show only 19% of exceptional species are stored across the MSBP and only 14% in the MSB itself (see Figure 1 & 2).
Alternative storage methods, such as tissue culture and cryopreservation, have been used to store specific genotypes/maternal lines for agricultural species. However, they are underutilised for wild flora conservation. Cryopreservation has successfully stored seeds of many exceptional species that can tolerate partial desiccation. However, many species’ seeds cannot be cryopreserved whole, so require use of alternate germplasm for storage. Currently, there is no one-size-fits-all solution, and optimum conservation requires species-specific protocols. After seed, the next best option is zygotic embryos; however, they require in vitro culture for recovery, an operation that can be time consuming, expensive, and dependent on a workforce trained in the techniques. When neither of the previous options work, clonal material can be used (dormant buds, callus tissue, shoot tips, somatic embryos etc). Clonal material, although a suitable option to conserve many exceptional species, significantly limits the potential for us to store and maintain genetically diverse ex situ collections. Complementary storage of desiccation-tolerant pollen alongside clonal material may be an option for restoring genetic diversity.
Developing new protocols for exceptional species is costly and time-consuming, hindering large-scale implementation. While waiting for progress of protocol development, initiating living collections can reduce the pressure on exceptional species in the wild, provide propagules for research, and maintain some genetic diversity ex situ in the short term. Living collections can also assist in situ conservation, which, if effective, may allow for some species to be declassified as exceptional. Any solution to conserve exceptional species will require collaboration and knowledge sharing. Even if developed methods have lower recovery success or store less genetic diversity, they are still better than storing nothing ex situ.
For the past couple of decades, the Millennium Seed Bank (MSB) has concentrated mainly on banking orthodox species and supporting global conservation efforts by sharing best practices through the MSB seed conservation standards and training for Millennium Seed Bank Partnership (MSBP) partners. With the success of banking orthodox species, the attention is turning to the subset of species identified as “exceptional”, i.e., unsuitable for long-term storage at -20°C, to reach future conservation targets. Different elements of this are to improve the survival of short-lived species, develop new methods for recalcitrant species by research, amended operation procedures and creating new training content.
In January, participants from Armenia, Bhutan, China, Greece, Indonesia and Israel visited the MSB for a one-week technical attachment focusing on the exceptional species. Some of the partners are already working on these approaches, and others have expressed an interest in learning more.
From collecting orchids at the right stage of seed pod maturity, as discussed in a talk from Jennifer Peach (MSB UK team), to the best techniques for cleaning seeds from fluffy Salix or spores from ferns, as demonstrated by the Seed collection team members responsible for microseeds, we covered methods already routinely used at the MSB. A guest talk from Dustin Wolkis of the National Tropical Botanical Garden from Hawaiʻi focused on different types of exceptional species and the strategies for their conservation.
All of the participants also shared their backgrounds and specific interests, for example, the challenging recalcitrant tree seeds of Indonesia (Dian Latifah) or the difficulties in growing the orchids of Bhutan (Pem Zam and Kezang Tobgay). One day of practicals with Dani Ballesteros was dedicated to an introduction to the cryopreservation of shoot meristem and embryonic tissues with methods like vitrification, droplet freezing and alginate encapsulation (for further information see Volk (2020) and Funnekotter et al. (2022)). These methods, as well as techniques on symbiotic orchid seed germination by Viswambharan Sarasan, are still part of research projects but will hopefully be further developed in the future.
It was great to share the new approaches with the course participants and we are planning to offer a more in-depth course on cryopreservation in October. Please contact MSBTraining@kew.org if you are interested in attending or to express interest in our annual Seed Conservation Techniques course which will be offered online this September.
In 2022 we undertook our biannual MSBP partner questionnaire to get your feedback on the MSBP and to help us shape the direction of the MSBP moving forward. The questionnaire was open from February to April and covered nine different areas related to seed conservation and the MSBP, including seed use, restoration, training, the MSBP Seed Conservation Standards, the MSBP Data Warehouse and the Samara newsletter.
In this article we provide an update on progress towards five key action points for ourselves that arose from analysis of the questionnaire results.
Scoping for an online workshop in 2023 covering some of the overlapping issues associated with seed distribution.
It was felt that some of the main barriers to seed distribution and use were: ensuring the correct paperwork was in place and legislation was followed, seed collection size, and the mechanisms and resources needed to undertake seed distribution. Whilst several of the issues which limit seed distribution are specific to individual countries, there are many areas which overlap between countries, hence this action point to scope the feasibility of running an online workshop in 2023, to help address some of these issues together. We have an internal discussion in the diary for early April on how best to take this forward, with further information to follow soon after.
Review feedback from the partner questionnaire, 2022 training courses and our online training resources to identify gaps and areas for improvement.
At the time of the questionnaire, in-person training had not resumed following the switch to online training during the COVID-19 pandemic. The results from the questionnaire indicated a general preference for in-person training with little support for an online-only approach. This feedback has already been taken into consideration with the resumption of in-person training alongside our review process for monitoring training feedback.. In addition, there were requests for training in specific areas, such as propagation and cryopreservation.
During 2022 our flagship Seed Conservation Techniques (SCT) course ran as a hybrid event with a week of online lectures and then two weeks of in-person sessions at the Millennium Seed Bank (MSB). We also delivered multiple in-country SCT courses, technical attachments at the MSB restarted, and we held a specialist exceptional species training course at the MSB (see article 'Special training for exceptional species'). Feedback from these courses indicated that they offered excellent training in areas highly relevant for participants’ job roles, that they were well organised, and were seen as good value for money (where course fees were applied).
Our current online training resources comprise 16 technical information sheets covering a range of ex situ seed conservation activities, from identifying desiccation sensitive species and assessing seed populations for collection, through to drying methods, germination testing techniques as well as seed bank design. A small number of technical videos and seed collecting manuals are also available.
Gaps were identified in our training provision, including areas such as seed use e.g., propagation and restoration training materials; specific details missing from training such as making agar plates, using a blue drum kit (for seed drying) and shipping guidance; and a need for more short-lived species guidance and resources. Another opportunity is to expand the range of technical videos available online.
Through the 2022/2023 onsite courses, filming took place and Hanna Oldfield (MSB Training Coordinator) has started developing additional resources, some of which will be available via the MSBP Data Warehouse website in the future. For 2023, the SCT course will be offered in an online format with individual training visits arranged for priority candidates. The aim is to alternative hybrid courses with online courses to balance the interest of candidates in practical training with the wider access and larger participation from online courses. Discussions are also underway for other training courses to be run in 2023 including a more in-depth course on cryopreservation in the autumn. Not all our training opportunities are advertised publicly, so if you have a specific seed conservation training need we would recommend speaking to your MSB Coordinator in the first instance or email MSBTraining@kew.org.
Improve our external communications around upcoming opportunities and new training materials.
The E-newsletter format of Samara allows us to be more dynamic with the information we share, and the ‘News and Events’ section is specifically designed to enable us to share updates when they become available. When the new training resources highlighted above are on the website they will be shared via this channel, as will forthcoming training opportunities. We also use the Kew website, social media channels and direct mailings to known training interested partners to advertise training courses.
Plan for the establishment of a panel to review potential updates to the MSBP Seed Conservation Standards.
There was a short break in running standards reviews due to staff changes and then the COVID-19 pandemic. The initial step to implement this action was to conduct a review of the Seed Conservation Standards once at least five reviews had been completed in 2022/2023. The aim of this was to give us a baseline of the standards process, and this review is currently underway. Once completed, we will use the information to plan the terms of reference for the panel and proceed from there. In addition to this we have also undertaken a Seed Conservation Standards review of the MSB.
Improve the visual appeal of the Samara e-mail and website.
The partner questionnaire highlighted the need to improve the visual appeal of the E-newsletter, both the website and the email. Hopefully you will have noticed some changes to the email this time round! There are now hyperlinks directly to each section and a generally more colourful appearance. We hope to improve this further in future, but must work within the limitations of our current software. For the Samara E-newsletter webpage we are restricted in terms of what we can do to improve the visual appeal, but have been including more photos and incorporating different styles of articles which was one of the great suggestions that came through the partner questionnaire.
Apodytes abbottii by Naomi Mdayi (SANBI)
Apodytes abbottii is a shrub or small tree, endemic to rocky sandstone outcrops in southern KwaZulu-Natal and north-eastern Transkei (Pondoland Centre). It was described as a new species from Southern Africa by Potgieter & Van Wyk in 1994 and is a range restricted species that occurs in a highly threatened habitat: Pondoland scarp forest, in forest margins, fire protected crevices and rock cliff faces above forested gorges. There are more than ten locations where the number of mature individuals is declining, due to too frequent and intense fires, and harvesting for firewood and building materials. As a result, A. abbottii is listed as Near Threatened (NT) on the Red List of Southern African Plants. The seeds of this species were collected by Fergy Nkadimeng in February 2022, near the Umtamvuna Nature Reserve. These seeds will be banked at the MSB for long-term storage.
Seed collection of threatened Leucadendron species "The Cone Bushes" by Naomi Mdayi (SANBI)
Leucadendron cinereum seeds, were collected at Riverlands Nature Reserve by Naomi Mdayi. She was joined by the two Kirstenbosch Gardens interns (Mane Somtshu & Thamsanqa Msweli), and they were accompanied in the field by one Cape Nature staff member from the reserve, for the two days they spent exploring the reserve. L. cinereum is a Proteaceae shrub, listed as Vulnerable on the Red List of South African plants, due to the declining and often endangered habitats where it occurs. Like other cone bushes, L. cinereum is dioecious, with insect-pollinated male and female flowers occurring on separate plants. Seeds are stored in small silver, fire-resistant cones. The plants are usually killed by fire, after which the wind-dispersed seeds are released from the cones. Another Vulnerable Proteaceae species, L. corymbosum was collected by Naomi Mdayi, Mashudu Nndanduleni (Kirstenbosch National Botanical Garden horticulturist) and Kirstenbosch interns, together with staff from Waterval Nature Reserve under Cape Nature. The seeds were collected at Voëlvlei Nature Reserve, another reserve managed by Cape Nature. This species occurs in the Renosterveld and sadly habitat loss continues to affect this and others, because of vineyard expansion, wetland drainage, urban expansion, alien plant invasion, road construction, road verge clearing and overgrazing. Leucadendron corymbosum seeds are not new to the MSB but were not collected in this region before and because of these ongoing threats, extra backup is essential.
Interviewed by Clare Callow (RBG Kew)
This month Michael Way offered the Samara team his insight into seed conservation and how to inspire the next generation of conservationists.
Michael joined Kew in 1993, originally as an ecologist with experience in Latin America, attracted by the idea of a seed collection position. He continues his focus on Latin America in his current role, and his remit now is on developing and running projects there, in his favourite part of the world.
Can you tell us a bit about your role?
I am based at Wakehurst and started working here in the era before the expansion of Kew Science when the biology of basic orthodox seed banking was increasingly well understood for wild species, and the plan to have an impact on the world stage had been piloted. We had one position for the Americas and one position for Africa, which had proven that it was possible to set up programmes of work from the UK through partners overseas. That came to be the business plan which was expanded into the truly global scale of the MSBP.
What does a typical day at work look like for you?
Most of my colleagues are based in the Americas, often six hours or more behind UK time, so my days are very much driven by this time difference. I normally start the day by looking at the overnight correspondence – queries from partners who are running our projects in Mexico, Colombia, or the USA, and then I will have an eye on our students and trainees – making sure their needs are met and following how their projects are developing. I’ll look at what needs organising - for our funded projects in terms of getting collections accessioned or inputting into projects in the development phase, and I'm increasingly trying to keep time for research and research support so that we are not just delivering work under existing protocols, we're actually trying to find better ways of doing our job in lots of different ways – scientifically, professionally, technically and logistically, wherever we can.
What influenced you to work in seed conservation?
I had experience in UK conservation before I went to Latin America, so I'd seen the negotiations between landowners and statutory bodies over the use of land, and the effect of funding organisations in this realm, so I understood the obstacles to nature conservation delivered just through land management. In Latin America I'd seen the amazing diversity of plants, and had worked in a small seed nursery for six months in Ecuador, which involved collecting seed from, and propagating, very threatened plants. The small NGO I worked with had responsibility for education and management in a cloud forest reserve just outside Cuenca, protected as a watershed for the city’s water supply.
What is your favourite aspect of your role at the moment?
We have recently greatly expanded our training programme, which has encouraged me to find novel ways of delivering our seed conservation knowledge to new audiences and to improve the way that we convey findings from research – not just taking place at the MSB but in different seed labs around the world – that is changing the way we think about handling seeds in the field, and presenting new options for working with exceptional species. The new Biodiversity and Conservation MSc programme that Kew has developed with Queen Mary University of London has provided a great opportunity for us to get restoration conservation and seed biology onto the curriculum, and help the next generation of students.
How do you see the work of the MSBP changing over the next decade?
I think day-to-day activities will become more digital as we pick up more tools to capture, share and analyse information faster and in real-time, but we are grounded in physical seed collections that need testing and propagating and using, and so there's a limit to how far digital tools are going to transform our day-to-day work. I think the nature of our relationships with partner organisations will continue to evolve - I'm thrilled that so many partners have had the opportunity to build their capacity through various programmes with Kew, and are now operating to a very high standard, are able to find funding and find new ways to do seed conservation in their different situations.
Working with students and interns gives us opportunities to shine lights on areas of work that we want to explore but perhaps wouldn’t be able to commit a whole new funded position to, which might lead to changes in the way we handle seed – for example, our current intern took the opportunity to collect Hedera helix (common Ivy) seeds from just outside the MSB in February. Those seeds are now being studied for desiccation sensitivity. This work will enable us to understand what that species requires, which wouldn’t have been possible without a programme of interns.
New exhibition at the Jardín Botánico "Viera y Clavijo" Seedbank
From the Jardín Botánico "Viera y Clavijo" Seedbank team:
This year we have inaugurated a new exposition in our Seedbank. This exposition is about the history of the seed (from the first plants with seeds 350 years ago to the present), the different dispersion systems (seed biology), the work in a seed bank (what we do), the biodiversity locked in a seedbank, seedbanks in the world, and threats to biodiversity.
More information about the Jardín Botánico Canario "Viera y Clavijo" can be found on their website.
Relaunch of the Seed Information Database (SID)
On the 23rd February 2023, the Society for Ecology Restoration's (SER) International Network for Seed-based Restoration (INSR) held a webinar to launch the new Seed Information Database.
More information on the launch can be found on the SER website.
If you have any recent publications (not just journal articles), please send them through to firstname.lastname@example.org so we can share them in upcoming issues.
Nadarajan, J., Walters, C., Pritchard, H.W., Ballesteros, D. & Colville, L. (2023) Seed Longevity - The evolution of knowledge and a conceptual framework. Plants. 12: 471. DOI: https://doi.org/10.3390/plants12030471
Sampayo-Maldonado, S., Ordoñ-Salanueva, C.A., Mattana, E., Way, M., Castillo-Lorenzo, E., Dávila-Aranda, P.D., Lira-Saade, R., Téllez-Valdés, O., Rodríguez-Arévalo, N.I.; Flores-Ortiz, C.M. & Ulian, T. (2023) Potential distribution of Cedrela odorata L. in Mexico according to its optimal thermal range for seed germination under different climate change scenarios. Plants. 12: 150. DOI: https://doi.org/10.3390/plants12010150
White, F.J., Ensslin, A., Godefroid, S., Faruk, A., Abeli, T., Rossi, G. & Mondoni, A. (2023) Using stored seeds for plant translocation: the seed bank perspective.Biological Conservation. 281: 109991. DOI: https://doi.org/10.1016/j.biocon.2023.109991
Issue 4: September 2022
In this issue we hear about field adventures in Svalbard and lab work on the endemic Lobelioideae of Hawaiʻi. As well as steps towards creating the third edition of the Red Book of the Republic of Azerbaijan, the progress of seed banking at Bali Botanic Garden and the creation of a Seed Production Garden on Kangaroo Island, Australia. This issue's interview is with Dr Dian Latifah, Coordinator of Seed Conservation Research Group within the National Research and Innovation Agency (BRIN), Indonesia. The seed question of the quarter looks at the difference between mouldy and infested seeds when doing cut-tests, X-rays and germination testing.
Stories from the field and lab this issue explore the Lobelioideae of Hawaiʻi and seed collecting in Svalbard.
The remote archipelago of Hawaiʻi in the Pacific Ocean is a hotspot of endemism with nearly 90% of the native flora being endemic to the islands, many being single-island or even narrower endemics (Sakai et al., 2002). Of the nearly 1,400 native plant taxa, nearly half are considered species of conservation importance and more than 230 plant taxa survive with fewer than 50 individuals remaining in the wild. There are several reasons for this endangerment, including habitat destruction, loss of pollinators, invasive species (including plants, rodents, and diseases), and climate change.
A diversity of complementary conservation measures are being taken to safeguard these plants, from seed banking and ex situ collections of plants in botanical gardens, to propagation and in situ out-planting in their natural habitat with varying success. Seed banks are fundamental resources for plant conservation, simultaneously providing a safety net through longer-term storage, as well as a means for linking ex situ conservation with in situ restoration and reintroduction. However, seed storage behavior varies greatly among species (Hong & Ellis, 1996) and understanding seed behavior is critical for successful seed banking and conservation. There is therefore an urgent need to understand evolutionary and ecological relationships with morphological and physiological seed traits that determine seed storage behavior.
While the majority of species are thought to produce orthodox seeds (i.e., desiccation and freeze tolerant), an estimated 8% of plant species are recalcitrant, or sensitive to desiccation (Wyse & Dickie, 2017). With some key families, life forms, biomes and levels of extinction risk having higher levels of non-orthodox behavior. In these instances, the proportion of species with recalcitrant seeds could be 40-70%. For those species that produce more desiccation tolerant seeds, there is a gradient of storage behavior, which includes species with intermediate storage behavior which show desiccation tolerance but poor storage at -20°C, where apparently orthodox seeds show a more rapid than expected decrease in viability over the course of a decade (Walters, 2015). Fully orthodox seeds can be expected to survive dry, cold storage for many decades.
Efforts to predict storage behavior based on measurable morphological or physiological seed traits (e.g., seed mass, lipid phase state changes, moisture content, and dormancy class) have thus far had limited success. This is particularly true for physiological traits that have historically been challenging to measure (like lipid phase state changes), but for which new technology has made measurements more accessible. Efforts to understand how seed storage behavior relates to ecological and phylogenetic relationships have been limited. Research has shown that storage behavior of seeds can be predicted by habitat and habit (Tweddle et al., 2003; Wyse & Dickie, 2017) and that there is a phylogenetic signal (Dickie & Pritchard, 2002) indicating storage behavior may be relatively conserved at low taxonomic levels. Moreover, there are suggested physical co-correlants of desiccation (in)tolerance, including seed mass and seed coat: whole seed mass ratio (Daws et al., 2006).
The endemic Hawaiian Lobelioideae (Campanulaceae; 6 genera, 126 spp.) exemplify adaptive radiation in plants and comprise the largest family in the flora of the most isolated archipelago on Earth (Wagner et al., 2005). Endemic Campanulaceae is distributed on seven of the eight main Hawaiian Islands ranging in elevation from <100 to >4000 m a.s.l. and demonstrates remarkable diversity of habit (e.g., shrubs, trees, rosettes, succulents, vines, epiphytes) and habitat (e.g., high elevation bogs, cliff faces, forests; Wagner et al., 1999). While the majority of species in the family produce orthodox seeds (i.e., desiccation and freeze tolerant), this is not the case with Hawaiian-endemic Campanulaceae which appear to have anomalous responses to conventional seed storage methods (i.e., equilibrium to 15-25% relative humidity (RH), and -18°C storage; Chau et al., 2019). Consequently, Hawaiian lobeliads provide an ideal model group for studying prediction of seed behavior though comparison with Campanulaceae globally.
As Seed Bank and Laboratory Manager at the National Tropical Botanical Garden (NTBG) headquartered on Kauaʻi, Hawaiʻi and a PhD Student at the University of Copenhagen Natural History Museum of Denmark, I seek to understand factors that affect seed longevity in the native Hawaiian flora. I recently traveled from Hawaiʻi to the UK for a research stay at RBG Kew’s Millennium Seed Bank (MSB) to study lipid thermal properties in the Hawaiian lobelioids and related groups. I targeted one representative species from each of the six Hawaiian clades from a recent global Lobelioideae phylogeny (Kagame et al., 2021), and one species from a closely related clade from the Marquesas from the NTBG Seed Bank and Lab collection, as well as one species each from three other closely related clades from the MSB collection. Unfortunately, I was not able to bring species from one of the Hawaiian clades outside of the United States as all taxa in it are federally listed as endangered in the US and permits could not be obtained. Supervised by Drs. Hugh Pritchard, Louise Colville, and Daniel Ballesteros, I used differential scanning calorimetry to capture the lipid thermal fingerprints for three replicates of each species, as well as performed annealing experiments at two temperatures. Somewhat serendipitously, on the last day of my visit, I was able to collect seeds of the critically endangered (possibly extinct in the wild) Delissea rhytidosperma (Walsh, 2015) on site, from the MSB research greenhouse, thereby capturing the final clade in the study that otherwise would not have been possible. By investigating the interactions between temperature and lipid melt and crystallization behavior over warming, we hope to gain insight into why these species exhibit poor storage behavior during conventional seed bank storage.
Chau, M.M., Chambers, T., Weisenberger, L., Keir, M., Kroessig, T.I., Wolkis, D., Kam, R. & Yoshinaga, A.Y. (2019) Seed freeze sensitivity and ex situ longevity of 295 species in the native Hawaiian flora. American Journal of Botany. 106: 1248-1270. DOI: https://doi.org/10.1002/ajb2.1351
Daws, M.I., Garwood, N.C. & Pritchard, H.W. (2006) Prediction of desiccation sensitivity in seeds of woody species: a probabilistic model based on two seed traits and 104 species. Annals of Botany. 97: 667-674. DOI: https://doi.org/10.1093/aob/mcl1022
Dickie, J.B. & Pritchard, H.W. (2002) Systematic and Evolutionary Aspects of Desiccation Tolerance in Seeds. In: Desiccation and Survival in Plants: drying without dying. Eds. Black, M. & Pritchard H.W. CABI publishing.
Hong, R.H. & Ellis, T.D. (1996)A protocol to determine seed storage behaviour. IPGRI Technical Bulletin No 1. Rome Italy: International Plant Genetic Resources Institute.
We have recently returned from the arctic, where we were carrying out the first year of fieldwork for a three-year project to conserve arctic flora. Funded by the Marris-Webbe Charitable Trust, the project will see seed collections made in three arctic locations – Svalbard (Norway), Disko Island (Greenland) and the Latnjajaure Lake area (Sweden). Research into the adaptability of tundra vegetation to climate change will be conducted, in addition to research looking at how resulting species and community level changes influence the functioning of arctic ecosystems. The Millennium Seed Bank (MSB) team will focus on the seed conservation aspects of the project, while Dr Anne Bjorkman’s team from the University of Gothenburg, Sweden will undertake the research and provide support with field sites and logistics.
Spitsbergen, the largest island in Svalbard, is not your typical summer destination – at 78°N, with forecast highs of 5°C, meltwater leading to boggy conditions and the promise of 24-hour sunlight, it sounded a long way from the heatwaves which enveloped Europe this year. The other fact that makes it stand out is that you cannot leave the main settlement of Longyearbyen (the world’s northernmost settlement) without a rifle and flares – to keep polar bears at bay.
The archipelago of Svalbard was completely ice-bound until 10,000 years ago, meaning that the vascular plant species found there have migrated relatively recently from Greenland, America, and Russia. The west coast of Spitsbergen (where the fieldwork took place) has a particularly favourable climate despite its latitude, as a result of the warm North Atlantic current.
Luckily it had been an early spring this year, meaning that seed set was good when we arrived at the end of July. Over eight days we managed to make 20 collections from the area surrounding Longyearbyen. These included the Svalbard Poppy (Papaver dahlianum), Arctic Cottongrass (Eriophorum scheuchzeri), and the Polar Willow (Salix polaris). The latter is one of only two ‘trees’ to occur in Svalbard, and has a low, creeping form less than 5cm tall – quite different from most Willow trees we know in the UK! Everything we collected was less than 20cm tall, which meant a lot of bending down and kneeling, and our main competition for the seeds seemed to be the hungry reindeer and geese that we often found nearby. Having explored higher up the valleys towards the glaciers and right down the coast of the fjord where beluga whales could be spotted, we found that most of the seeds were (not surprisingly) found in the valley bottoms and lower slopes, and along the coast. The fewer plants found growing higher up had not yet had time for their seeds to ripen due to later snow melt and cooler temperatures.
Our collections, and their accompanying herbarium specimens will soon arrive back in the UK for us to process and store. Half of each seed collection and an herbarium specimen will be kept at the National Seed Bank of Norway, in the Botanical Garden in Oslo, to ensure the safety of the collections through duplication.
Across the MSBP articles this issue come from Azerbaijan, Indonesia and Australia.
The wild flora of Azerbaijan is extremely rich and diverse, with more than 5,000 species of higher plants. Azerbaijan's natural vegetation and its flora are exposed to anthropogenic influences, and the number of valuable plant species is decreasing, with some now threatened with extinction. This is gradually having a negative effect on the ecosystem. Our latest research shows that at least 10% of the higher plants of Azerbaijan are rare and endangered. There are many relicts as well as endemic plants of the Caucasus and Azerbaijan which are collected from their natural habitat for use in the production of food, fodder, medicine, spices or decorative items and have thus become rare. To address this, in recent years, the Institute of Botany has been carrying out research on the ex situ protection of endangered plants, as well as developing the intraspecies systematics reflecting the gene pool of those species; studying limiting factors under in situ conditions; including investigating the reasons for the increase of the limiting factors, and ecological assessments. Ecosystem management requires a comprehensive strategic approach, which means studying and protecting the coexistence of land, water, and wildlife, in other words, all living systems.
An important factor for the detection and documentation of rare and endangered plants is the preparation of Red Books and Red Lists. In 2013, the 2nd edition of the Red Book of the Republic of Azerbaijan (300 plant species) was published. The results of scientific research show that the number of plants to be included on this list may now have doubled. To help identify the species that need protection, monitoring and expeditions are organised in different regions of the country based on lists compiled by Azerbaijani botanists ahead of the publication of the 3rd edition of the Red Book of the Republic of Azerbaijan, due to be published in 2023. Two further books have already been written - "Red Book of Nakhchivan Autonomous Republic" and "Rare plants of Ganja-Kazakh region". In order to preserve these studied species, information about the species is collected and action plans for in situ and ex situ conservation are prepared. It is especially important to study the rich flora and biodiversity of the Karabakh territory, characterised by its climate and landscape diversity. There are 121 rare Azerbaijan and Caucasus endemics found across Karabakh, which is famous for its unique flora but has remained unstudied for 30 years due to conflict. Most of these plants will need to be added to the 3rd edition of the “Red Book”.
The Institute of Botany and the Millennium Seed Bank Partnership have been cooperating bilaterally for 10 years to protect biodiversity. The main goals of the partnership are the implementation of joint new projects, the exchange of personnel and experience, and joint research on priority areas. So, as a result of currently implemented joint projects, the seeds of approximately 40% of the rare and endangered higher plants included in the 2nd edition of the Red Book of the Republic of Azerbaijan have been collected. These collections are stored in the Seed Bank of the Institute of Botany and duplicated at the Millennium Seed Bank and are important for the protection of those species in the future and for the implementation of restoration and reintroduction work over the coming years.
Since its opening in 2006, the collections at Bali Botanic Gardens’ seedbank mainly came from garden plants (Lestari & Asih, 2015). Recognising the need to increase genetic diversity for restoration and re-introduction purposes (Rao et al., 2006), collecting wild plant seeds began on Nusa Penida in 2018, and then in Bali Barat National Park in 2019. The seedbank also started to receive seed collections from Bali Botanic Gardens’ flora exploration teams at that time.
However, seed harvest and collection management efforts at Bali Botanic Gardens’ seedbank still did not entirely comply with the standardised seed banking procedures recommended by Kew’s Millennium Seed Bank. Increasing the capacity of staff at Bali Botanic Gardens was seen as a way to address this condition which might otherwise hamper the seedbank’s conservation effort. The capacity improvement need was highlighted further by the fact that in 2016, Bali Botanic Gardens seedbank had become part of the collaboration between the Indonesian Botanical Gardens Seedbank and the Millennium Seed Bank Partnership (MSBP).
Staff from Bali Botanic Gardens attended several capacity building activities hosted by the MSBP Kew team. The first was the regional Seed Conservation Techniques course held in Cibodas Botanic Gardens from 25th - 29th April 2017 (Figure 1). In the same year, a member of staff from Bali Botanic Gardens attended the 19-day Seed Conservation Techniques training course held at the MSB, Kew. These courses were very beneficial as the material and practical activities were provided directly by the Millennium Seed Bank teams.
Seed banking capacity improvements at Bali Botanic Gardens needed to reach as many people as possible. Transferring knowledge to one or two staff members would not have been practical for the long term as staff at the seedbank are continuously changing. Thus, in September 2020 Bali Botanic Gardens seed banking staff met with others from all Indonesian Botanic Gardens to share and discuss their experiences in seed collection following MSBP standards. As travelling at that time was restricted due to COVID-19, the meeting was held virtually. The meeting gave staff valuable insight into seed and data collection processes to be used during and after field trips. However, confusion and uncertainty remained in the minds of staff as most had never used these processes first-hand.
As a follow up, two seed and data collection practice sessions were conducted in October 2020, with the objective to provide staff with a glimpse of what they would do during the seed collection process. Both sessions were held in Bali Botanic Gardens, first with the Proteaceae collection and then with the Legumes collection (Figure 3). Although the first session was pretty challenging, the second went more smoothly as the staff were already familiar with the procedure and forms that would be needed in the field.
In 2021, nine Bali Botanic Gardens staff participated in a virtual seed banking training session held by the MSBP, in partnership with various stakeholders in Indonesia. For this training, a small number of staff participated together in the office, to support those unfamiliar with virtual meetings (Figure 4).
The training was very beneficial and provided the staff with the basic knowledge needed to maintain collections in the seedbank, including how to conduct seed viability tests, collection data management and other aspects regarding seed collection maintenance. The training also provided an opportunity for the staff to interact with the MSBP team, discuss and clear all confusion regarding seed banking procedures. The training gave staff a much more comprehensive understanding of the seed collection process and know how to maintain their collection in the seedbank.
We hope that the capacity strengthening conducted over the years will improve the standard of the Bali Botanic Gardens’ seed banking efforts in the future. We also hope that by involving a wider group in the capacity building activities, including staff not currently posted at the seedbank and a university student (at the second field practice), we will ensure the longevity of the seed banking efforts.
Lestari, D. & Asih, N.P.S. (2015)Pengelolaan bank biji Kebun Raya Eka Karya Bali. Proc. Masy. Biodiv. Indonesia. 1(3): 515-520.
Rao, N.K., Hanson, J., Dulloo, M.E., Ghosh, K., Nowell, D. & Larinde, M. (2006)Manual of Seed Handling in Genebanks. Biodiversity International, Rome.
The Australian Black Summer of 2019-20 saw the western half of Kangaroo Island, South Australia burnt by intense bushfires. More than 167,000 hectares were affected, largely comprised of remnant bushland, with 83% of the area burnt at high to very high severity. Kangaroo Island, along with the adjacent Mt. Lofty Ranges, is one of Australia’s 15 ‘Biodiversity Hotspots’ – concentrated areas of high biodiversity characterised by elevated levels of endemism.
The team from the South Australian Seed Conservation Centre (SASCC) arrived on the island shortly after these catastrophic fires to assess the post-fire response of some of the island’s endemic and threatened flora and, where possible, collect germplasm for long-term storage at the Botanic Gardens and State Herbarium, South Australia. Sensing the community’s desire to not only restore what was lost during the fires, but also to be better prepared for the next fire event, the SASCC devised the novel concept of a ‘seed production garden’.
A grant delivered through the Australian Seed Bank Partnership, funded by the Commonwealth Government of Australia, was used to develop the Kangaroo Island Seed Production Garden (SPG). The vision is to create an aesthetic garden of genetically diverse, living plant collections that can be used for conservation, restoration and translocation, population supplementation, research and seed banking, and is supported by the local community, benefactors (Paton Family), business (Troppo Architects) and several environmental NGOs (Bio-R, Nature Conservation Society of South Australia). An important aspect of this community-led garden will be to introduce members of the local community and visitors to the island’s threatened flora. Targeted seed production will also bridge an existing gap in the restoration of self-perpetuating populations of many threatened plant species on the island by ensuring the availability of sufficient, genetically robust and sustainably sourced propagules.
The development of the SPG is the result of almost two decades of seed collecting trips to the island by the SASCC team. The island boasts 60 endemic plant species, many of which were already germplasm-banked at the Botanic Gardens in Adelaide at the time of the fire. The SASCC was able to utilise some of this material – often collected when wild populations were larger and most likely had higher levels of genetic diversity – to propagate plants for introduction to the SPG. Excess propagules were also distributed to conservation-minded community members to augment existing populations or to establish new populations.
While the concept of seed production areas is nothing new, the idea of marrying utilitarian seed production with an inviting, aesthetic garden setting may be. Removed from the abstractions of formal reports and scientific literature, viewing a diverse collection of threatened plants up close and in a single convenient location makes the germplasm collection and propagation aspects of plant conservation tangible and hands-on. Rather than traversing dense vegetation to reach an isolated swamp in a remote Wilderness Protection Area at the western end of Kangaroo Island, a volunteer or visitor can witness the splendour of the Vulnerable, midday blooming aquatic Nymphoides geminata (Menyanthaceae), collect seeds from dehiscing fruits and propagate plants for translocation – all just 20 minutes from the island’s major township of Kingscote.
The half-hectare garden has been fenced to exclude browsing kangaroos, wallabies and possums and is landscaped with different soils and water features to match the habitats of the species being grown. On 9th July 2022 the SPG was officially opened by the State Minister for Climate, Environment and Water, the Hon. Susan Close MP, in the company of dozens of community members. During the opening the first 1,500 plants comprising over 60 threatened and endemic species were planted into the landscaped beds by eager volunteers.
At present, only one quarter of the garden has been planted, with enormous scope for the inclusion of additional species, multiple provenances and in-ground germination research. The SASCC team is now working on harnessing the public interest that the development of the garden has received into a ‘Friends of the Kangaroo Island Seed Production Garden’. This group will have a large ongoing role in the operation of the garden. The SASCC will provide technical guidance, including training volunteers in seed collection techniques, plant propagation and searching for new wild populations of target species. After what was for many Kangaroo Island residents a traumatic event, it has been wonderful to see people’s energy focused towards what will hopefully be a long-lived and substantive contribution to plant conservation in South Australia.
During a visit by MSB coordinators to Indonesia in August this year, they interviewed Dr Dian Latifah about her experiences of how she got into seed banking, her job and what are the challenges for seed banking in Indonesia.
How did you become interested in plant conservation?
Since I was a kid I loved growing plants in our garden, and my parents loved it too. That influenced me to take my BSc in Agronomy at IPB university. I did my BSc thesis on the seed science of rice. Then I applied to LIPI to be a seed scientist working on wild plants. I’ve been doing research on seed conservation since then.
My MSc and PhD were also on seed science, this time on palm regeneration of wild palms in Queensland. I spent 5½ years in Australia.
How has seed conservation at Bogor Botanic Garden (BBG) changed since you began working?
When I started working at Bogor Botanic Gardens, they collected orthodox seeds from the gardens, didn’t measure their moisture content and stored them at room temperature. Our director at the time, Dr Didik Widyatmoko (now he is a research professor), wanted to improve seed bank procedures, so my colleagues the late Mr Sutrisno, Dr Siti Roosita and I wrote a proposal to get state funding for seed bank development. After we got the funding, I searched online to find where I could do training and discovered the Millennium Seed Bank (MSB). I contacted the MSB and attended the Seed Conservation Techniques course in 2015, and our partnership developed from there.
Why is seed conservation important to BRIN (Research Centre for Plant Conservation, Botanic Gardens and Forestry at National Research and Innovation Agency of the Republic of Indonesia) and Indonesia?
At the start, the strategy for ex situ conservation, consisted of living collections and seed banking. Both have pros and cons. E.g., Living collections need more space, but the seed bank only needs a little space, and we can store a much greater diversity of plants. Therefore, we focused on both. We follow GSPC Target 8 that 75% of a country’s biodiversity should be stored ex situ in-country, and seed banking contributes to that. From the start, we have been building the collections through field work, prioritising rare and endemic plants, and those with economic potential, for example, Myristica spp. (nutmeg) and Eusideroxylon zwagerii (ironwood).
A seed bank is a big investment, and we need to show value for money. We do this through increasing collaboration with students, academics and the public.
Indonesia is a very large country, with lots of islands. You have two big seed conservation projects with the MSB. How do you organise your teams to collect from the different parts of Indonesia?
At the start I asked for proposals from my colleagues at the four botanic gardens in Bogor, Cibodas and Purwodadi (Java) and Eka Karya (Bali) and submitted them to Kew. We now have 11 sub-projects covering different geographic regions, aiming to collect over 1,000 species, under the Arcadia Threatened Biodiversity Hotspots project. This project lasts for three years until 2024 and each year we organise field trips to different islands, from Sumatra to Papua. We chose several National Parks and Nature Reserves that have high biodiversity and where we have built up a good relationship with the authorities
What are the challenges for seed collecting in tropical forests?
(Laughs). First, we are dealing with remote areas and challenging weather – it rains a lot so it may limit the time available for seed collecting and reduce access to the areas. There is also a lack of information on fruiting seasons – we may get some advice from the park rangers, but when we arrive the fruits are immature. Then there is often a lack of information on seed storage behaviour on references like the Seed Compendium, other articles, SID and the Wyse predictor – sometimes we are not sure if a species is orthodox or not, and when we try to do storage or germination experiments it seems to be recalcitrant. It is difficult to get enough seeds, up to 10,000. On average, we often can only get from 250 to 3,000. We may only be able to find a small number of trees, often less than five, and the ones we can find may be inaccessible. Finally, we need up to seven different letters and permits with named target species to collect and conserve seeds from protected areas and it may take weeks to get them, so we have to start planning well in advance of each trip.
How would you like the Kew-BRIN partnership developing in the future?
We would like to extend the project in Mt Ciremai National Park, to achieve more significant results. The current project, funded by the Garfield Weston Foundation, is a combination of seed conservation and utilisation for restoration, that involves several parties, including Mt Ciremai National Park, Kuningan Botanic Gardens / Kuningan District Council, BRIN and Kew. The National Park has an ecosystem restoration programme underway, so our project will support this. We can involve Kuningan Botanical Garden as they have nursery skills. BRIN and KEW bring additional technical and scientific resources and help provide training and develop facilities.
The Arcadia project will end in 2024 and we would like to do more research on the seeds we have collected and monitor their viability, to learn more about the seed storage behaviour and longevity. For example, we have found problems with conservation of Begonia seeds, many of them are endemic and also have economic potential as amenity species. We’d also like to do more projects, such as setting up seed hubs in other parts of Indonesia for local seed storage and use.
What is the most interesting plant you're working on at the moment?
Eusideroxylon zwagerii (ironwood) is an important species as it’s endangered and its timber is very high quality and has great economic value. It is known to have recalcitrant seeds, so we have been doing research on its seed storage behaviour and the best method of short-term storage. We can now store its seeds for up to three years!
Mouldy versus infested seeds? Scoring X-rays and germination tests
Can an X-ray detect fungal, bacterial or viral infections in a seed? When should seeds be classified as 'mouldy' or 'infested' in a germination test? Do insect infestations differ from fungal, viral or bacterial infections when scoring germination tests? We asked Sarah, Nicola and Rachael from the Millennium Seed Bank (MSB) Seed Collections Team to talk us through the differences.
Can you spot seeds infested with fungus on an X-ray? And how should these be scored?
At the seed quality checking stage of seed processing, a seed counted as 'infested' can relate to either insect damage/ presence or a bacterial/ fungal/ viral infection.
Whilst an X-ray cannot always tell us if a seed is infected with a fungus, bacteria or a virus, sometimes it can be seen for example, as a darker grey 'shadow', spreading out across the seed from one point. This often has a vaguely granular texture rather than the pure white of a healthy seed. A seed can also be counted as 'infested' at this stage if the infection is so severe it is clear that the seed is non-viable from the outside. Or for some smaller seeded species, for example with many Scrophulariaceae, the contents can still appear solid on an X-ray. In these cases, you can do a cut-test instead of an X-ray to determine the internal morphology and quality.
The signs of insect infestation to look for on X-rays are tunnels, usually of increasing size, with a cavity at the end where the lava may be (Figure 1a). In some cases, a seed may have been almost entirely eaten by an insect, in which case the seed will appear as almost empty with a few lighter spots of content (likely frass) left behind (Figure 1b). Insect infested seeds also sometimes visibly show exit holes for larva on the outside of the seed coat, for example with some Fabaceae species. However, it should be noted that insect infestation does not necessarily mean that the seed is inviable, it can depend on how much of the seed has been consumed. At the MSB there is a ‘part full’ category alongside ‘full’, ‘empty’ and ‘infested’ for scoring X-rays which can be used if there is some uncertainty as to whether enough of the seed embryo remains (has not been eaten) for the seed to still be viable.
Should those seeds which are black at the end of the germination test be recorded as 'mouldy' or 'infested'?
NB. When you are setting up a germination test, if you have any concerns about the seeds, you can do a cut-test on a sub-sample prior to setting up the test.
At the MSB we use six different categories to classify seeds during a germination test (Table 1). But when you’re scoring your test as you go along or are doing your cut-test at the end, you may have some degree of fungal growth and it is not always clear under which category the seeds should be classified.
Table 1: Germination test seed classifications
Include in germination calculation?
Included as 'germinated'
Included as 'not germinated'
Included as 'not germinated'
Included as 'not germinated'
'Infested' varies from 'mouldy' in that a seed should be classified as 'mouldy' if you can see signs that there was suitable initial morphology at the start to enable germination, i.e., an embryo was present (Figure 2a). If this is not possible to detect the seed should be classified as 'infested' (Figure 2b). If a seed is classified as 'mouldy' it could suggest that the seeds have either lost their viability over time, there was poor test hygiene, or that the germination conditions were incorrect, e.g., too warm.
Is it possible for fungal spores to spread from one seed to another during storage?
It would be possible for a fungus to spread between seeds during the cleaning process if equipment is not cleaned properly between uses or if the seeds come into close contact with an infected batch whilst drying. Once the seeds are dried and sealed for banking, assuming they are fully sealed, it is less likely for fungal spores to transfer between collections. The other possible source of fungal spores for infection could be the medium used for germination testing, e.g., agar, or from the air whilst setting up the test. If you suspect seeds could be infected with fungal spores, it is possible to sanitise the seeds prior to starting the germination test. This can be done by soaking the seeds in 0.5% sodium hypochlorite (NaOCl) solution, containing a 1% surfactant (Tween 20) for 10 minutes and then rinsing under running water for 1 minute (see Technical Information Note 13a). Once the germination tests are set up, the container they are in should be sealed inside a plastic bag to reduce the chance on contamination (this also helps to prevent moisture loss). In addition, 'mouldy' and 'infested' seeds should be removed from the test as it progresses to limit the influence they have, and tests can be re-plated during the test if fungal growth gets too bad.
In this issue we hear about field adventures in Madagascar and Zambia, research studies from Georgia, Italy and the UK, and about the latest edition of the Australian Plant Germplasm Conservation guidelines. We interview Naomi Carvey, MSBP Data Warehouse Officer at the MSB and the species profile is the US endemic Daucosma laciniata.
Madagascar is committed to achieving Target 8 of the Global Strategy for Plant Conservation, and in the spirit of any post-2020 targets, we wish to ensure that all our threatened flora is conserved, and available as ex situ material for future conservation, restoration and research. For MSBP Madagascar this means, we want to rapidly increase the number of threatened species conserved as seed collections, where possible. The Kew MSBP Madagascar team, in collaboration with our national partners, the Silo National des Graines Forestières (SNGF) is implementing the Global Tree Seed Bank Programme and we have adopted a new collection strategy based on geographical gaps in our current collections. An analysis of the collection density over the years has given us a clear picture that the majority of the collections made so far, have been from outside protected areas. To compensate for this spatial anomaly, field trips carried out during 2022 are focussed on protected areas, in order to cover as many sites as possible to ensure the collection and ex situ conservation of species of restricted distributions, including large numbers of more threatened species.
Initially, six potential sites located in the northern part of Madagascar were chosen, four of which were new to the MSBP. These are the Kasijy, Ankarana, Andrafiamena Andavakoera and Analamerana Protected Areas. Each site has its own climatic and geographical specificities that affect the distribution of the different types of vegetation. There is xerophytic vegetation on the Tsingy (Ankarana, Montagnes des Français Protected Areas), deciduous dry forests (Kasijy, Analamerana, Andrafiamena Andavakoera, Ankarana Protected Areas) and degraded dry forest in the rocky hills around Ambilobe.
In Madagascar, accessibility to many sites by vehicle is almost impossible especially during the rainy season, and this has been made worse as a result of the cyclones we have witnessed over the past few years. The team's fieldwork in January and March coincided with the passage of two cyclones in the north eastern part of the island, which subsequently led to heavy rains in the region. This has greatly affected our fieldwork plans. For example, the visit to Andrafiamena Andavakoera had to be abandoned due to the rise of the Irodo rivers that have to be crossed to reach the site. Our Landrovers are good, but 6ft deep rivers in spate are a match for any wading vehicle!
The team had a memorable 14-day collection trip recently to Kasijy. From our base it is a two day drive to the regional headquarters where we spent the next day securing access and collecting permits. We then had a two and a half day trek simply to reach the forest edge. The subsequent four days spent collecting yielded 14 collections, all new for us. I am very proud of the team commitment this effort showed.
Working in new sites is a big challenge especially with a poorly known flora like Madagascar. A lot of the information we have for our target species does not correspond with what we find on the ground. Predicting fruiting periods and seed maturity based on herbarium data has not been very successful and for many sites, we do not have the network of collaborators on the ground to monitor phenology. We are also witnessing a wide variation in periods of seed maturity between species at a particular site. Securing seed from many species on one site visit is therefore, a challenge. Many of these protected areas are void of roads and paths and it has proven very difficult to assess the total population size for our target species.
Of the 82 collections made during these six collection missions, 11 are thought to be new to the MSB collections and 19 are threatened species.
Among the many interesting trees harvested during these field trips was Psydrax sambiranensis. Assessed as Critically Endangered (CR) and known only at one site in the rocky hills around Ambilobe district, it is threatened by rock mining for construction. With the post harvest work of SNGF, all these seed collections have now been cleaned and are ready for duplication to the MSB for long-term storage.
Daniellia alsteeniana P.A.Duvign. locally known as Mukulabushiku in our Bemba language is a conspicuous and very ornamental tree with large pink flowers growing to some 25m. It belongs to the Caesalpinioideae sub-family of legumes, and in the Northern parts of Zambia it is considered a threatened species through over-exploitation for its timber and resin. It is highly valued by local people providing construction materials for housing, canoes, crates and decorative veneer.
Currently the species is Near Threatened on the IUCN Red List and whilst we recognise that large, healthy populations of the species are still to be found in Angola and the DRC, due to its over-exploitation in Zambia we made this a priority for our collection programme. We intend to make seed material much more widely available for the many restoration projects currently being launched amongst these Miombo woodlands in Zambia. Lab-based germination reaches 98% and seeds are easily raised in field nurseries.
Herbarium vouchers stored at one of the largest and oldest Zambian National Herbariums (NDO) located in Kitwe, show plant specimens collected in Kasama (1957) and Kawambwa (1958) in Northern and Luapula provinces of Zambia, respectively. These 70-year-old records suggested fruiting occurred between October and November but following our collection trips to these areas in 2019, we found the seed was actually at the point of natural dispersal during August and September. Was this change in fruiting time something to do with our changing climate or simply a response to variation in the time of the rainy seasons? Our collection will add to our understanding of the fruiting behaviour of this species. We now have a good herbarium voucher, useful field images and a modest seed collection of some 3000 seeds stored here in Zambia with a safety duplicate at the MSB. We intend to revisit these provinces in the coming seasons to bulk-up these numbers with new collections.
The species dispersal mechanism is interesting. Each fruit (pod) contains 1-2 bi-convex oval and very glossy brown seeds each weighing about 4g. The funicle is persistent and so it seems to be the detached valve of the pod that falls, like a whirling samara, thus assisting dispersal of the attached seeds beyond the shadow of the crown.
Modern plant taxonomy considers blackthorn (P. spinosa) as a conservative species. Fundamentally, phylogenetic niche conservatism refers to the tendency of species to retain their ancestral traits. There is a substantial difference in the interpretation of this term by Russian and European schools of botany. The floras of the USSR and post-soviet countries are rich in plant species with morphological, and in some cases, even phenological characteristics which vary over short distances across their spatial range.
The floras and plant nomenclatural checklists of the Black Sea countries of Ukraine, Moldova, and Turkey suggest the existence of blackthorn species, subspecies, and variations that share nearly the same ancestral traits as P. spinosa. These are: P. podolica Andrz. ex Trautv.; P. spinosa var. dasyphylla (Schur) Domin; P. kurdica Fenzl ex Fristch; P. spinosa subsp. dasyphylla (Schur) Domin; P. stepposa Kotov. Most of them, except for P. spinosa subsp. dasyphylla, are synonyms of P. spinosa according to the Global Biodiversity Information Facility (GBIF). The old edition of the flora of Georgia (Sosnovsky, 1949; Gulisashvili, 1965) and the modern updates of the plant nomenclatural checklists for the country (Gagnidze et al., 2005; Davlianidze et al., 2018) indicate the distribution of the single taxon P. spinosa in the country. However, the local botanist N. N. Bregadze discovered five sub-taxa (all considered synonyms in GBIF) within the local population of this species:
The description of the morphological characteristics of these varieties and forms is provided in the study “The Botanical and systematical research of the diversity of Prunus spinosa L.” (Botaniko-sistematicheskoe Izuchenie Prunus spinosa L. in Russian) published in 1976. The study was soon followed by a series of more comprehensive studies on the diversity of the Prunus genus L. in the South Caucasus conducted by another local botanist Eugenia Baiashvili who revealed the existence of differences in the chromosome number of individuals of P. spinosa in the country. The compilation of her articles on this topic was published as a monograph in 2010 (Baiashvili, 2010).
The discovery of the higher level of intraspecific variability inspired us to conduct a case study where we researched the population diversity of blackthorn in Georgia with the use of the modern methods of morphometry and plant genetics. The research was carried out as part of the “Enhancing Rural Caucasian Community Livelihoods Through Fruit and Nut Conservation” project and responded to its general aim of collecting and interpreting information about the globally and locally threatened species to raise local public awareness about the diversity of these key groups of plants.
The morphological study revealed three clusters of individuals, two clusters (clusters A, B; Fig. 1) weakly separated individuals of P. spinosa on the basis of the height of the shrub and leaf size i.e., these clusters included large and small-sized shrubs which are not associated with specific habitats or geographic sites in Georgia with their distribution. The third cluster (Cluster C, Fig. 1) strongly separated morphologically distinct forms with oval-shaped leaves and elliptic fruits and fruit stones - generally distributed in drylands of the Samtskhe-Javakheti region in the south part of Georgia (Fig.'s 1 & 4).
The results of the genetic study showed weak divergence both on the intra and interspecific levels in the studied samples which supports the assumption that the species emerged after quick evolutionary radiation in the plum genus. Despite the low resolution of the genetic divergence of the taxa in the studied groups, the results supported the data of the morphometric study by proving the existence of the difference in haplotype structure between the group of individuals of P. spinosa distributed in the south part of Georgia and the rest of the individuals of this species collected in the other parts of the country.
Our results support the observation of the local scientists N. N. Bregadze and E. Baiashvili, and provide a more complete understanding of the diversity of blackthorn and its distribution in the country. We plan to continue the research in the future to discover the diversity of the plum genus at larger geographical scales in the Caucasus. With the taxonomical and ecological aspects of the research, it is important to focus also on the collection of ethnobotanical information. Fruits of blackthorn are collected in all parts of the Caucasus by local people because of their gastronomical and medicinal properties. They are even sold in the small countryside and large markets in Georgia. In this respect, it will be interesting to find out if local people differentiate blackthorn fruits by taste or any other property as this information would serve as additional proof of our findings on the existence of morphological and genetic differences between the sub-populations of P. spinosa.
The research was carried out within the framework of the project “Enhancing Rural Caucasian Community Livelihoods Through Fruit and Nut Conservation” funded by the Darwin Initiative Foundation in collaboration with the National Botanical Garden of Georgia, the Botanical Institute of Ilia State University, Georgia, and Royal Botanic Gardens, Kew from 2017-2021. As a result of this study, an MSc thesis with a similar title to this research was defended by A. Kvlividze at the Ilia State University in Georgia on February 18, 2022.
We are grateful to the project managers: Aisyah Faruk, Ian Willey, and David Kikodze, and Dr. Tsira Mikatadze-Pantsulaia - Head of the Plant Conservation Department at the National Botanical Garden of Georgia, for supporting this research.
Baiashvili, E. (2010)Phylogenetic study of genus Prunus L. (‘Kliavis (Prunus L.) Gvaris Philogenetikuri Shestsavla). Ed. Akhalkatsi, M. ; Botanical garden and the Institute of Botany. Tbilisi, Publishing house ‘Universal’. 130 pp.
Davlianidze M., Gviniashvili Ts., Mukbaniani M., JinjoliaImnadze L., Jugheli, T. (2018)Nomenclatural checklist of the flora of Georgia (Sakartvelos Phloris Nomenklaturuli Nuskha). Tbilisi, Publishing house ‘Universal’. 298pp. (in Georgian).
Gagnidze R.I., Maier G., Nakhutsrishvili G.S. (2005)Vascular Plants of Georgia: A Nomenclatural Checklist. Tbilisi, Georgian Academy of Sciences. 247pp.
Gulisashvili, S. [Ed.] (1965). Flora of the woody plants of the Caucasus, vol.: 4 (Rosacea-Leguminosae)(Kavkasiis Dendrophlora, Tomi IV (Rosacea-Leguminosae)). The Academy of Sciences of Georgian USSR, Institute of Forestry. Tbilisi, Publishing house ‘Metsniereba’. 405 pp. (in Georgian)
Sosnovsky, D. (1949) The Flora of Georgia, vol.: 5 (Rosacea-Leguminosae) (Sakartvelos Phlora, Tomi: V (Rosacea-Leguminosae)). Ed. Kharadze, A. Publishing and press of the Academy of Sciences of Georgian USSR, Tbilisi. 134 pp. (in Georgian)
Limited availability of data relating to regeneration traits has curbed macroecological research into the germination niche (Saatkamp et al., 2019). However, seed banks that focus on the ex situ conservation of wild species routinely carry out research to identify seed dormancy and germination requirements (Hay & Probert, 2013), which have become a valuable source for large-scale studies into seed germination (Sentinella et al., 2020).
In Europe, the European Native Seed Conservation Network, known as the ENSCONET Consortium, was established in August 2010 with the aim of maintaining significant levels of ex situ seed conservation activity for European native plant species. It builds on the success of the ENSCONET project, funded under the European Commission’s Sixth Framework Programme for Research and Technological Development (FP6) between 2004 and 2009 (Eastwood & Müller, 2012). The objective of the initial project was to improve quality, coordination and integration of European seed conservation practice, policy and research for native plant species and to assist EU conservation policy and its obligations to the Convention on Biological Diversity and its Global Strategy for Plant Conservation (Müller, Eastwood & Linington, 2012). A total of 33 institutes across Europe currently constitute the ENSCONET Consortium, all of which have an explicit interest in seed conservation of European native plants. One of the successful outputs has been the establishment of the ENSCONET database (ENSCOBASE), an online database that has been storing data on European native seed accessions since 2005, covering all European geographical regions and major plant families (Rivière et al., 2018).
ENSCOBASE contains publicly available primary records of laboratory germination experiments from multiple seed banks across all European Biogeographical Regions. It holds information on experimental germination conditions (germination temperature, diurnal temperature regime, light and dormancy-breaking treatments), alongside seed viability records and the associated seed lot metadata (e.g. biogeographical region; Fig. 1). As such, ENSCOBASE represents a valuable source of information to understand the macroecology of seed germination. Yet, there are challenges to the analysis and interpretation of the data, mainly due to the inherent heterogeneity related to each seed bank employing differing seed processing protocols. This between-study variation, in terms of meta-analysis theory can be addressed by applying recent developments in phylogenetic meta-analytical methods (Garamszegi, 2014). Based on this approach, we recently conducted a meta-analysis of the germination data stored in ENSCOBASE (Carta et al., 2022), finding that climate, specifically drought and cold seasons, shape the germination niche of temperate plants. The methodology used was able to account for between-study variation relating to seed sources and physiological status, thereby highlighting how large datasets generated by conservation seed banking can be a valuable resource in addressing questions in plant macroecology and evolution. We started the process in 2018, and although initially we found the heterogeneity of the original data difficult to manage, the resulting experience and eventual outcome proved to be a worthwhile and impactful study of the European flora.
Carta, A., Fernández-Pascual, E., Gioria, M., Müller, J.V., Rivière, S., Rosbakh, S., Saatkamp A., Vandelook F. & Mattana, E. (2022) Climate shapes the seed germination niche of temperate flowering plants: a meta-analysis of European seed conservation data. Annals of Botany. mcac037. DOI: https://doi.org/10.1093/aob/mcac037
Eastwood, R. & Müller, J.V. (2012) Achievements of the European Native Seed Conservation Network – ENSCONET. In: Maxted, N., Ehsan Dulloo, M., Ford-Lloyd, B.V., Frese, L., Iriondo, J., Pinheiro de Carvalho, M.A.A. Eds. Agrobiodiversity conservation: securing the diversity of crop wild relatives and landraces. pp. 286-291. Wallingford. CABI.
Garamszegi, L.Z. (2014)Modern phylogenetic comparative methods and their application in evolutionary biology: concepts and practice. Heidelberg; London. Springer Verlag.
Hay, F.R. & Probert, R.J. (2013) Advances in seed conservation of wild plant species: a review of recent research. Conservation Physiology. 1(1): cot030. DOI: https://doi.org/10.1093/conphys/cot030
Müller, J.V., Eastwood, R. & Linington, S. (2012) ENSCONET: a milestone for European seed conservation. Studi Trentini de Scienze Naturali. 90: 209–210.
Rivière, S., Breman, E., Kiehn, M., Carta, A. & Müller, J.V. (2018) How to meet the 2020 GSPC target 8 in Europe: priority-setting for seed banking of native threatened plants. Biodiversity and Conservation. 27: 1873-1890. DOI: https://doi.org/10.1007/s10531-018-1513-2
Saatkamp, A., Cochrane, A., Commander, L., Guja, L.K., Jimenez-Alfaro, B., Larson, J., Nicotra, A., Poschlod, P., Silveira, F.A.O., Cross, A.T., Dalziell, E.L., Dickie, J., Erickson, T.E., Fidelis, A., Fuchs, A., Golos, P.J., Hope, M., Lewandrowski, W., Merrit, D.J., Miller, B.P., Miller, R.G., Offord, C.A., Ooi, M.K.J., Satyanti, A., Sommerville, K.D., Tangney, R., Tomlinson, S., Turner, S. & Walck, J. et al.(2019) A research agenda for seed-trait functional ecology. New Phytologist. 221(4): 1764-1775. DOI: https://doi.org/10.1111/nph.15502
Sentinella, A.T., Warton, D.I., Sherwin, W.B., Offord, C.A. & Moles, A.T. (2020) Tropical plants do not have narrower temperature tolerances, but are more at risk from warming because they are close to their upper thermal limits. Global Ecology and Biogeography. 29(8): 1387-1398. DOI: https://doi.org/10.1111/geb.13117
Text description of Figure 1: Map of European Biogeographical Regions
A map of Europe showing the areas covered by 11 different biogeographical regions. The Alpine biogeographic region covers most of Norway, and is also present in the Pyrenees, the Alps, the Carpathian Mountains, the Balkan Mountains, the Caucasus Mountains, the Ural Mountains and a small section of the Apennines in central Italy. The Anatolian biogeographic region covers the central area of Turkey. The Arctic biogeographic region covers Iceland, Svalbard, Novaya Zemlya and northern Russia. The Black Sea biogeographic region covers extends around the coast of the Black Sea from Bulgaria, through Turkey and into Georgia (west to east). The Atlantic biogeographic region covers the United Kingdom, Ireland, northern Spain, westerns parts of France and Denmark, Belgium, the Netherlands and northern Germany. The Boreal biogeographic region extends from Sweden east covering Finland, Lithuania, Latvia, Estonia, northern Belarus and western Russia. The Continental biogeographic region extends from western France through southern and eastern Germany, Czechia, Poland, southern Belarus and northern Ukraine into Russia, also extending south into northern Moldova, eastern Romania and Bulgaria, Serbia, Bosnia and Herzegovina, northern Croatia and north-west Italy. The Mediterranean biogeographic region extends from Portugal and Spain, east into southern France, western areas of Italy and coastal areas of Croatia, Bosnia and Herzegovina, Montenegro, Albania, Greece and Turkey. The Pannonian biogeographic region covers Hungary, north into Slovakia and south into northern Serbia. The Steppic biogeographic region extends from western Romania along the Black Sea coast into southern Ukraine and Russia
The third edition of ‘Plant Germplasm Conservation in Australia – strategies and guidelines for developing, managing and utilising ex situ collections’ was recently released by the Australian Network for Plant Conservation (ANPC) and the Australian Seed Bank Partnership (ASBP) (Fig. 1). These ‘Germplasm Guidelines’ are practical, technical and evidence-based, providing a workflow to address each step of acquiring, maintaining and utilising genetically representative collections (Fig. 2).
These Guidelines bring together decades of research and experience in conserving Australian plants in seed banks, botanic gardens and conservation nurseries (Fig. 3). They take readers through the genetics and practice of acquiring collections and the processes of seed banking, tissue culture, cryopreservation, and living collections maintenance, with 50 case studies to highlight the application of research and theory. In this edition, we add new chapters on the role of the nursery and identifying and conserving non-orthodox seeds. We also address the need to capture and utilise ‘special’ types of germplasm (material from ferns, mosses, liverworts), or taxa with ‘special’ life history stages or growing requirements (terrestrial orchids with mycorrhizal associations, carnivorous and parasitic plants). With an eye to the future, we finish with new chapters on risk management, and collection maintenance and utilisation. We gratefully acknowledge the input of 78 contributors from across the restoration and agriculture sectors, botanic gardens, the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and universities, as well as the Kew Millennium Seed Bank, Botanic Gardens Conservation International, the Center for Plant Conservation and the Exceptional Plants Conservation Network to update this edition; and thank The Ian Potter Foundation for funding this project.
The Germplasm Guidelines were launched by Prof. Tim Entwisle during the Australasian Seed Science Conference in September 2021. This virtual meeting tackled issues across seed biology and ecology through to the sourcing, end-use and management of seed collections, as well as the roles that seeds play in local and global culture. As a fully-virtual meeting the conference attracted 425 delegates from 34 countries, enabling researchers to share their work, including the new edition of the Germplasm Guidelines with an engaged international audience. Additional funding from the Australian Academy of Science is enabling the ANPC to continue these discussions through the Australian Academy of Science Fenner Conference on the Environment: ‘Exceptional times, exceptional plants’.
Exceptional plants include those species for which seeds are not available (not produced at all, inadequately produced, or non-viable), species with non-orthodox seeds (desiccation sensitive, freezing-sensitive or short-lived), and species with deeply dormant seeds. While the work of seed banks is important, and although the majority of seed-bearing plant species are considered to be orthodox, we still face challenges with many species when it comes to determining how to store them longer-term in ex situ conservation facilities and regenerate them from seed.
Many conventional seed banks are already working on exceptional species in collaboration with botanic gardens, nurseries and other experts to identify alternative options for conserving exceptional plant species. The 3rd edition of Germplasm Guidelines presents examples from around Australia through several informative case studies and workflows which may be of assistance to facilities that have recently started or are planning to commence work on exceptional species.
The written guidelines are supported by high-quality video content accessible via the 'Plant Germplasm Conservation in Australia' playlist on the ANPC YouTube channel.
Text description for Figure 2: Decision flowchart for ex situ conservation options
A flowchart for deciding ex situ conservation options, referencing the relevant chapter and section in the Germplasm Guidelines.
The taxon or population is considered at risk or in need of ex situ conservation (Chapter 1) leading to the questions (Section 2.3):
Have all major considerations been addressed?
Is there a clear link to an in situ recovery process?
Is it a precautionary collection?
Is it for research or display?
Is it an ‘exceptional species’? (Chapter 6)
What is the time frame?
What information is available?
Is seed available (preferred in most cases)?
Will you need to conduct preliminary trials on collection, storage or propagation?
Do you have the necessary resources? Can you get the necessary permits or permissions?
Using these it is then asked do the benefits of ex situ conservation outweigh the risks (Section 2.2)? If no, monitor populations or pursue other options, if yes decide ex situ options. If seed is not suitable, consider other options (Section 2.4, Chapter 8). Then collect material appropriate to the options chosen (Chapters 3 and 4).
Collected material can then be used in four different ways:
Seed banking (Chapters 5, 6 and 7)
Tissue culture, orchid and symbiont culture (Chapters 9 and 12)
Cryopreservation (Chapters 10 and 13)
Living plant collections (Chapter 11)
Material from these four options can then be utilised (for translocation, research etc.) and appropriate risk management implemented (Chapters 14 and 15).
Text description for Figure 3: Location of major ex situ conservation facilities for the Australian flora
A map of Australia split by state showing the locations of the major ex situ conservation facilities for the Australian flora:
In the Northern Territory:
George Brown Darwin Botanic Gardens conservation seed bank which has seedbanking and nursery production facilities
Alice Springs Desert Park which has seedbanking and nursery production facilities
In Western Australia:
Western Australian Seed Centre, Department of Biodiversity, Conservation and Attractions, Kensington, and Kings Park and Botanic Garden which has seedbanking, cryostorage, nursery production and micropropagation facilities
Forest Products Commission Seed Centre which has seedbanking and nursery production facilities
In South Australia:
The Australian Pastures Genebank, South Australian Research and Development Institute, which has seedbanking and nursery production facilities
the South Australian Seed Conservation Centre, Botanic Gardens and State Herbarium of South Australia (BGSH), which has seed banking and nursery production facilities
The Australian Grains Genebank, Agriculture Victoria, which has seedbanking and nursery production facilities
The Victorian Conservation Seedbank, Royal Botanic Gardens Victoria, which has seedbanking, nursery production and micropropagation facilities
The Tasmanian Seed Centre, Sustainable Timber Tasmania, which has seedbanking and nursery production facilities
The Tasmanian Seed Conservation Centre, Royal Tasmanian Botanical Gardens, which has seedbanking, nursery production and micropropagation facilities
In New South Wales:
The National Seed Bank, Australian National Botanic Gardens, which has seedbanking, nursery production and micropropagation facilities
The Australian Tree Seed Centre, CSIRO, which has seedbanking and nursery production facilities
The Australian PlantBank, Australian Institute of Botanical Science, Royal Botanic Gardens and Domain Trust, which has seedbanking, cryopreservation, nursery production and micropropagation facilities
The Brisbane Botanic Gardens Conservation Seed Bank, Brisbane Botanic Gardens, Mt Coot-tha, which has seedbanking and nursery production facilities
Wild edible plants (WEP) are, for the most part, uncultivated and have one or more edible parts. These plants can be well-known in the area where they are found and used, but often, the knowledge of how to use them has been lost.
Despite the large number of WEP reported (> 7,000 species, Diazgranados et al., 2020), only 30 are currently part of our diet, with more than 50% of our calorie intake based on wheat, rice, maize and sugar consumption (FAO, 2020). These few dominant crops have been bred over millennia, drastically narrowing their genetic diversity in comparison to wild species. Crop wild relatives (CWRs) are species closely related to crops, that are used as a source of genetic diversity to improve conventional crops. According to Castañda-Álvarez et al., (2016), there are more than one thousand CWRs registered, of which only 150 have their conservation status assessed (IUCN). In many cases, CWRs exhibit tolerance and resistance to biotic and abiotic stresses that can be transferred to crops via breeding techniques.
Many CWRs are threatened in the wild and need to be prioritised for ex situ collection to conserve their diversity. This is most pressing for endemic species, which may have unique genetic and phenotypic traits that could be lost if their habitat is vulnerable (e.g., to climate or land use change). Storing CWRs in seed or gene banks not only ensures their conservation but also makes material accessible for research into the traits that they possess.
Several collaborative projects between the Millennium Seed Bank (MSB) and its partners have undertaken seed collection of WEP, gathering more than 3,000 WEP (>15,000 accessions, Fig. 1). Furthermore, the MSB Partnership (MSBP) has collected and conserved CWRs from at least 25 crops (mainly pulses, cereals and forages listed in Annex I of the International Treaty on Plant Genetic Resources for Food and Agriculture) to investigate their characteristics and select key accessions for crop improvement purposes. Nearly 300 CWRs species (3767 accessions) were collected during 2010-2020 from 90 countries (Fig. 2).
Despite the extraordinary work and extensive efforts of the institutions involved, WEP and CWRs continue to need urgent collection and conservation (Vincent et al., 2019, Maxted and Vincent, 2021). More than 50% of WEP and CWRs are still under-represented in genebanks and little knowledge is available for most of them. In response, a gap analysis was performed to identify key species that need to be prioritised for conservation, as well as regions or hotspots with a high number of WEP and CWRs not yet conserved across the MSBP. The objective is to ensure their long-term conservation, to increase the diversity and accessibility of collections.
Southeast Asia and Malesia, together with West and West-Central Tropical Africa were identified as the regions with the greatest number of WEP not yet conserved (Fig. 3). On the other hand, Southeast Asia and Latin America, more specifically Brazil, were identified as hotspots for CWRs that are not stored ex situ (Fig. 4). We recommend that the top five genera with missing collections for WEP (Elaeocarpus, Pinanga, Pandanus, Agave and Garcinia) and CWR (Artocarpus, Arachis, Solanum, Brassica and Prunus), together with other endemic taxa, should be targeted from the aforementioned regions, establishing new partnerships if needed, for conservation efforts. This will help secure the genetic diversity held in these species for current and future generations.
Castañeda-Álvarez, N.P., Khoury, C.K., Achicanoy, H.A., Bernau, V., Dempewolf, H., Eastwood, R.J., Guarino, L., Harker, R.H., Jarvis, A., Maxted, N., Müller, J.V., Ramirez-Villegas, J., Sosa, C.C., Struik, P.C., Vincent, H. & Toll, J. (2016) Global conservation priorities for crop wild relatives. Nature plants. 2(4): 1-6. DOI: https://doi.org/10.1038/nplants.2016.22
Diazgranados, M., Allkin, B., Black, N., Cámara-Leret, R., Canteiro, C., Carretero, J., Eastwood, R., Hargreaves, S., Hudson, A., Milliken, W., Nesbit, M., Ondo, I., Patmore, K., Pironon, S., Turner, R. & Ulian, T. (2020)World checklist of useful plant species. RBG Kew. DOI: https://knb.ecoinformatics.org/view/doi:10.5063/F1CV4G34
Food and Agriculture Organization of the United Nations (2020)FAOSTAT Statistical Database. Rome: FAO. (accessed May 2022)
Genesys (2022). Data accessed through Genesys Global Portal on Plant Genetic Resources, www.genesys-pgr.org, (accessed Jan 2022).
Maxted, N., & Vincent, H. (2021) Review of congruence between global crop wild relative hotspots and centres of crop origin/diversity. Genetic Resources and Crop Evolution. 68(4): 1283-1297. DOI: https://doi.org/10.1007/s10722-021-01114-7
Seed Bank Database, SBD, (2021)(accessed Jan 2022)
Vincent, H., Amri, A., Castañeda-Álvarez, N. P., Dempewolf, H., Dulloo, E., Guarino, L., Hole, D., Mba, C., Toledo, A. & Maxted, N. (2019). Modeling of crop wild relative species identifies areas globally for in situ conservation. Communications Biology. 2(1): 1-8. DOI: https://doi.org/10.1038/s42003-019-0372-z
Text description for Figure 1: Distribution of wild edible plant accessions conserved ex situ at the MSB.
A world map highlighting high numbers of wild edible plant population accessions held at the MSB from the United States, Mexico, Mali, Burkina Faso, Sudan, Tanzania, Botswana, South Africa, Madagascar, Italy, UK, China and Australia.
Text description for Figure 2: Distribution of CWR accessions conserved ex situ across the MSB.
A world map highlighting high numbers of crop wild relative plant population accessions held at the MSB from the United States, Mexico, Ecuador, Chile, Ghana, Nigeria, Sudan, Uganda, Kenya, UK, Spain, Italy, Israel, Syria, Jordan, Turkey, Pakistan and Vietnam.
Daucosma laciniata Engelm. & A.Gray, also known as Meadow Parasol or Meadow Daucosma, is a herb native to Texas and New Mexico in the United States (Lady Bird Johnson Wildflower Center, 2022; USDA, NRCS, 2022). It was first described to science in the Boston Journal of Natural History (volume 6, pg. 211) by Asa Gray in the article “An account of a collection of plants made by F. Lindheimer in the western part of Texas, in the years 1845-6, and 1847-8, with Critical Remarks, Descriptions of New Species” (IPNI, 2022). Digitised versions of the herbarium sheets from this collection are available through the Harvard University herbaria and RBG Kew herbaria.
Daucosma is a genus within the Apiaceae (Umbelliferae) family, with Daucosma laciniata being the only species of the genus (WCVP, 2022), making it taxonomically distinct. The species has had its genome sequenced as part of the Plant and Fungal Tree of Life (PAFTOL) project (available at: https://treeoflife.kew.org/specimen-viewer/10171).
The species is an annual, flowering between June and July with white umbellifer flowers (Lady Bird Johnson Wildflower Center, 2022). Its current conservation status is G4 - Apparently Secure (NatureServe, 2022a), which means the species is at fairly low risk of extinction (NatureServe, 2022b).
One collection of the species is held at the Millennium Seed Bank (MSB), donated by the Lady Bird Johnson Wildflower Center in 2005. Since 2006 various germination tests have been performed on the collection without achieving significant germination. However, cut-tests at the end of these experiments revealed that the seeds were still alive, indicating that the perfect dormancy breaking conditions for the species remained elusive, rather than the collection being inviable . In 2019, Kirstine (Germination Assistant at the MSB) set up various tests based on a combination of the best conditions from previous germination test results and WORLDCLIM average monthly temperatures for the area the collection was made. Fast forward 504 days (72 weeks!) and one of the tests reached 92% germination (100% viability after accounting for empty and viable ungerminated seeds). The germination conditions consisted of 4 steps:
Firstly, 8 weeks cycling at 20°C for 12 hours, then 10°C for 12 hours with 12 hours of light each day.
Secondly, 16 weeks at 5°C with 12 hours of light a day.
Thirdly, 14 weeks cycling again at 20°C for 12 hours and then 10°C for 12 hours with 12 hours light a day.
And finally, 34 weeks cycling at 35°C for 12 hours and 20°C for 12 hours with 12 hours light a day.
This germination protocol still needs to be refined, although this will probably take another year and a half! However, it does show that the seeds are viable, and we have been able to break the dormancy of this particular collection which will allow for the propagation of the plants from seed in the future.
Gray, A. (1850). An account of a collection of plants made by F. Lindheimer in the western part of Texas, in the years 1845-6, and 1847-8, with Critical Remarks, Descriptions of New Species. Boston Journal of Natural History. 6:211. Available online from the Biodiversity Heritage Library website
This month we spoke with Naomi Carvey, Millennium Seed Bank Partnership (MSBP) Data Warehouse Project Officer at the Millennium Seed Bank (MSB). Naomi has one of the most wide-reaching roles within the Seed Conservation team at the MSB and will have been in contact with many of you. So we thought we’d take the opportunity to introduce the person behind the email address!
What did you do before joining the MSB?
I’ve always had data and systems type roles in my career prior to joining Kew, but in very different industries. My first full-time job after leaving University was pricing flights and holidays for a big travel company, after that I started working in banking in the City of London, including working for several years at the Bank of England – the UK central bank. Before that, whilst I was still in full-time education I spent several years milking cows and helping with wedding catering during weekends and holidays – something quite different!
Why did you join the MSB and what interested you about the role?
While still working in banking and studying GIS I became interested in environmental datasets and applications. I heard about the new MSBP Data Warehouse project first, and through that rather stumbled across the wider work of the MSBP. I thought it sounded like a really interesting and dynamic place to work, and the role seemed to suit my experience and interests well.
What is your current role and what does a typical day look like for you?
I’ve been the MSBP Data Warehouse Project Officer for several years now, helping to gather, improve and secure data from seed collections across the MSB partnership, and to get the data back into the MSBP community. A typical day might involve checking and feeding back on a dataset from an MSB partner, and then uploading those data to the MSBP Data Warehouse online. I might also be updating IUCN red list data or species names, helping colleagues with data analysis or delivering data training.
Can you tell us about something you are proud to have achieved in your role?
Recently I have worked on the design and implementation of processes that allow us to receive herbarium vouchers from MSB partners who do not duplicate their seed collections at the MSB, but share full collection and curation data instead. I have found this work to be very enjoyable and rewarding. Duplicating herbarium vouchers at RBG Kew for seed collections stored elsewhere throughout the partnership really opens up fantastic possibilities for research and collaboration, as well as elevating seed collections to the highest quality standards, maximising collection utility and longevity.
What are some of the challenges you have faced, or think you might face in the future?
Increasingly I feel there is a gap between the fantastic wealth of seed collections, their associated data and new research done around the world, and getting those seeds and their associated knowledge back into living ecosystems. There is some amazing work being done across the MSBP in restoration and on new techniques in habitat management but currently issues around seed supply, a lack of germination / cultivation protocols and the difficulties transporting germplasm across borders are holding this work back. I think plant conservation should be a cycle of gathering collections and knowledge and putting them to practical use, but there are some bottlenecks at the moment. I’d love to get more involved in work to help tackle some of these issues.
Can you tell us about a favourite moment from your work for the MSBP?
Meeting colleagues from other seedbanks across the MSBP is always a highlight – we work with such a fantastically diverse group of people, and it is always great to learn about associated projects, not to mention discovering more about plants! The annual Seed Conservation Techniques (SCT) course is always special and I’ve also been lucky enough to visit some MSB partners in-country. Each visit has been really beneficial, and I have life-long memories of them all. To highlight one, the SCT course held at Cibodas Botanic Gardens in Indonesia in 2017 – the course had a significant impact on the future direction of the collaboration and a large number of people were able to join the training, which was very well organised and held in such beautiful surroundings. Through Arcadia and the Global Tree Seed Bank programme, the National Research and Innovation Agency (BRIN) - our MSB partner in Indonesia – is now working on collecting over 1000 species.
What advice would you give somebody regarding their data?
Concentrate on completing any mandatory fields first, and be prepared to invest a significant amount of time on your data at the beginning and during data collection – if you do a thorough job at the start you will save a lot of time and many headaches later on! You will also be able to maximise the longevity and potential future use of your collections. Don’t be afraid to ask for help from your colleagues at the MSB - we have a lot of varied experience and resources and we’re here to help.
If you could be one plant, what would you be and why?
I’d be a beautiful, craggy old Yew tree, Taxus baccata, a tree known for its cancer-fighting chemicals, toxicity and folkloric associations with death and resurrection which are sometimes thought of as portals to the underworld. In the UK ancient Yew trees are often found in churchyards and some pre-date the arrival of Christianity. I have fond memories of collecting T. baccata seeds for the UK National Tree Seed project - my first ever seed collecting trip for the MSB which was highly influential for me.
How many seeds should I collect?
How many seeds to collect is a question of balance between making sure you can gather enough seeds for your intended use in the time you have available, while ensuring you do not harm the population from which you are sampling. In general, for a long-term conservation collection, you should aim to collect at least 10,000 potentially viable seeds, but not taking more than 20% of the available mature seed. Population data should be used to guide when more cautious collecting might be necessary, e.g., when making a collection from annual species that produce few seeds. The recommendation to collect 10,000 potentially viable seeds is based on the seed use figures in Table 1, which were calculated from collections of abundant species held at Kew, and is a starting point that can be adjusted for specific projects or collection events. You should also keep in mind the importance of maximising the genetic diversity captured, by collecting randomly from as many individuals (maternal lines) as possible.
Table 1: Indicative breakdown of seed numbers for different uses from a single collection (adapted from: MSBP Technical Information Sheet 02, RBG Kew).
Indicative Seeds Required
Base collection kept in case of loss of wild population
Ideally 500 seeds
Developing an effective germination protocol
Viability monitoring over the anticipated 200-year life span of the collection
Duplication at another bank for safety
At least 1,150 seeds
Distribution to users
5,000 seeds (sufficient for 100 researchers to receive a 50 seed sample)
Future propagation and restoration projects
At least 2,500 seeds for trialling
The recommendation to collect 10,000 seeds might need to be adapted depending on the quality of the seed you are collecting. You will need to undertake a cut-test to verify the quality of seed from your sample population. Usually you need to cut-test ten seeds. If two of those ten are empty (20%), you can assume that 20% of the seeds you collect will be empty, therefore to collect 10,000 potentially viable seeds you would need to collect 12,000 seeds (10,000 x 1.2). You will then need to calculate again whether it is possible to collect this quantity while still ensuring you are taking no more than 20% of the available mature seed.
In the case of rare and threatened species, what is the minimum sized collection that is useful for a seed bank? There is no absolute threshold but ideally you should aim for a collection of 500 seeds. However, this will not always be possible and it is important to consider other factors, for example:
does the safe-collecting limit need to be lower?
how many seeds are available?
what are the intended end uses of the seed?
In cases where it is not possible to collect sufficient seed in a single visit, repeating collections over multiple years may be an alternative option.
Carta, A., Fernández-Pascual, E., Gioria, M., Müller, J.V., Rivière, S., Rosbakh, S., Saatkamp A., Vandelook F. & Mattana, E. (2022) Climate shapes the seed germination niche of temperate flowering plants: a meta-analysis of European seed conservation data. Annals of Botany. mcac037. DOI: https://doi.org/10.1093/aob/mcac037
Carta, A., Mattana, E., Dickie, J. & Vandelook, F. (2022) Correlated evolution of seed mass and genome size varies among life forms in flower plants. Seed Science Research. 32: 46-52. DOI: https://doi.org/10.1017/s0960258522000071
Hardstaff, L.K., Sommerville, K.D., Funnekotter, B., Bunn, E., Offord, C.A. & Mancera, R.L. (2022) Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat. Plants. 11(8): 1017. DOI: https://doi.org/10.3390/plants11081017
Pirie, M.D., Blackhall-Miles, R., Bourke, G., Crowley, D., Ebrahim, I., Forest, F., Knaack, M., Koopman, R., Lansdowne, A., Nürk, N., Osborne, J., Pearce, T.R., Rohrauer, D., Smit, M. & Wilman, V. (2022) Preventing species extinctions: A global conservation consortium for Erica. Plants, People, Planet. First view. https://doi.org/10.1002/ppp3.10266
Rodríguez-Zúñiga, J., Flores-Ortiz, C.M., González-Guillén, M.d.J., Lira-Saade, R., Rodríguez-Arévalo, N.I., Dávila-Aranda, P.D. & Ulian, T. (2022) Cost analysis of seed conservation of commercial pine species vulnerable to climate change in Mexico. Forests. 13: 539. DOI: https://doi.org/10.3390/f13040539
Tiloca, G., Brundu, G. & Ballesteros, D. (2022) Bryophyte spores tolerate high desiccation levels and exposure to cryogenic temperatures but contain storage lipids and chlorophyll: understanding the essential traits needed for the creation of Bryophyte spore banks. Plants. 11:1262. DOI: https://doi.org/10.3390/plants11091262
MSBP Technical Information Sheet Update
The Technical Information Sheets available on the Resources page of the MSBP website have recently been updated and republished. The Technical Information Sheets cover various aspects of seed conservation practices and facilities for example:
As part of these updates all the pdf's have been made web accessible. If you have any issues using the Technical Information Sheets, please contact email@example.com .
Issue 2: March 2022
This issue we hear about field, lab and training activities from Mozambique, Australia and Indonesia. We interview Victoria Wilman, Seed Conservation Programme Manager at SANBI (South African National Biodiversity Institute) and this month's species profile comes from Indonesia - the Begonias native to the Mount Slamet region. We also check back in with the Seeds of Hope featured in the news section of the December issue of Samara.
Nationally endemic plants are very important in conservation planning as their survival depends on only one country. Icuria dunensis (Fabaceae) is not only an endemic species to Mozambique, but also one of Mozambique’s five endemic genera (Darbyshire et. al., 2019a). It is listed as Endangered on the IUCN Red List of threatened species (Darbyshire et. al., 2019b) and its distribution is limited to a stretch of about 200 km along the Mozambican coast between Nacala (Nampula province) and Moebase (Zambezia province).
Icuria trees can grow to 40 m tall. They are dominant in patches of dry coastal forest, sometimes forming pure stands (Burrows et. al., 2018). The fruits are velvety woody pods about 10 cm long, with only 1-3 large seeds inside.
Importance of the tree for communities
Icuria trees are valued by local communities. For example, in the Mulimone Icuria forest in Nampula province, trees are debarked for canoe making (Fig. 1) and the wood is used for construction and firewood. However, trees are also killed by ring-barking a section of the trunk to clear the land for agriculture (Fig. 1). In addition, clearance of vegetation during the mineral sand mining occurring in this region, threatens the remaining patches of these Icuria forests.
Orthodox or Recalcitrant Icuria dunensis seeds?
In its habitat we noticed many Icuria trees completely without, or with very low numbers of, mature fruits. In order to guarantee enough quality seeds of Icuria for restoration purposes there is an urgent need for ex situ conservation by seed banking. Very little is known about the seeds of this species. They are large and fleshy with a thin seed coat, which suggests they may be recalcitrant.
Preliminary seed tests were conducted using seeds collected by Kenmare in Nampula Province, Larde district, in January 2019. The tests were carried out at the IIAM-CIF laboratory (Fig. 2). To determine the seed moisture content, fresh seeds were cut into small pieces using secateurs and divided into two samples of 10 grams. The samples were dried in the oven at 130°C for an hour. The fresh seed weight was about 87 seeds/kg, with a moisture content of 34.5% (IIAM-CIF, 2020).
124 seeds of I. dunensis were sown in sterilised sand in the germination room, with a temperature between 25-30°C, 24 hours photoperiod and air relative humidity between 60-80%. The seeds started germinating between day 3 and day 21. The germination was epigeal, with the cotyledons forcing above the surface of the germination medium as the seedlings grew (Fig. 3).
The 91% germination result was very encouraging and exceeded our expectations. However, this was just the start of our Icuria seed conservation research. Further tests are required to check if the seeds are indeed sensitive to desiccation, and to determine how germination responds to drying (Gold and Hay 2014).
Production of seedlings
The seedlings were pricked out, transplanted into polyethylene pots and placed in the nursery under shade. Five months later, the seedling survival rate was 100% and the seedlings reached 30-40 cm tall (Fig. 4). This is encouraging as it shows that if fresh seeds are available, it may be easy to produce seedlings for restoration and ex situ conservation.
Test if the seeds are desiccation-sensitive using the seed coat ratio model and seed storage behaviour protocols (Gold and Hay 2014);
Send duplicate seeds to the MSB for further seed testing including germination testing after banking;
Reinforce/strengthen the in situ conservation and restoration programme for Icuria in partnership with Kenmare and other potential partners.
This work was carried out under the MoU between IIAM-Kenmare to restore Icuria dunensis and implement in situ conservation initiatives. To be able to continue our Icuria seed research we appreciate the support of the Global Tree Seed Bank and Threatened Biodiversity Hotspots programmes that are funding Mozambique’s National Seed Conservation Programme at IIAM.
Darbyshire, I., Timberlake, J., Osborne, J., Rokni, S.; Matimele, H., Langa, C., Datizua, C., de Sousa, C., Alves, T., Massingue, A., Hadj-Hammou, J., Dhanda, S.; Shah, T. & Wursten, B. (2019a) The endemic plants of Mozambique: diversity and conservation status. Phytokeys. 136: 45-96. DOI: https://doi.org/10.3897/phytokeys.136.39020
Darbyshire, I., Massingue, A.O., Osborne, J., De Sousa, C., Matimele, H.A., Alves, M.T., Burrows, J.E., Chelene, I., Datizua, C., Fijamo, V., Langa, C., Massunde, J., Mucaleque, P.A., Rokni, S. & Sitoe, P. (2019b)Icuria dunensis. The IUCN Red List of Threatened Species 2019: e.T136532836A136538183. DOI: https://dx.doi.org/10.2305/IUCN.UK.2019-2.RLTS.T136532836A136538183.en (Accessed on 02 February 2022)
IIAM-CIF (2020, Report). Relatório sobre testes de qualidade de sementes de Icuria dunensis fornecida pela Kenmare-REHAB.
The work of the Australian Seed Bank Partnership (ASBP) is built on collaboration. Our relationship with Kew’s Millennium Seed Bank Partnership (MSBP) has been instrumental to our success in building capacity to deliver seed banking projects over the past 20 years. Following the devastating 2019–20 Australian bushfires, the MSBP reached out once more to help us secure our threatened flora. Thanks to a very generous offer of support from the UK Foreign, Commonwealth and Development Office (FCDO) in February 2020, we have been able to undertake an emergency collecting programme in impacted areas during the first season post-fire.
Target species for this programme were selected based on the severity of bushfire impact, their threatened status, and their potential to provide critical ecosystem services that support Australia’s native wildlife. Our partners made 47 collections of 42 taxa and delivered 164 germination trials of 93 taxa, representing flora from every Australian state and territory. On Kangaroo Island, two rare orchids (pictured) were located and banked by the South Australian Seed Conservation Centre. The funding provided for this programme has also enabled us to propagate 10 priority plants in Western Australia, Victoria and South Australia for planting in seed production areas and botanic garden displays.
To date, ASBP partners have also undertaken rapid flora assessments for 11 bushfire affected flora to ascertain habitat condition, initial fire response, species abundance, pests and disease presence and overall fire impact. To ensure standardised data collection across multiple states, our partners developed a methodology for species monitoring. This programme funded the printing of dedicated field books to capture this information. The assessments have provided seed banks with crucial information about species recovery post-fire. The data will be uploaded to botanic gardens databases and made available to those involved in research, conservation, and bushfire recovery actions to monitor recovery over time and inform future seed collection priorities.
Despite unforeseen project delays due to COVID restrictions, flooding, and impacts of the current La Niña event, the Partnership has adapted to ensure an ongoing commitment to ex situ conservation of Australian flora. This includes adjusting our species target lists to collect other accessible priority species, postponing our activities to the 2021–22 summer season, and establishing seed orchards from previous collections where species cannot be feasibly located.
The work our partners have delivered under this project, and many like it, mean thousands of native species are now secured in seed banks throughout Australia. We hope our efforts and the ongoing interest from beyond our shores will inspire people to learn more about protecting our native plants in our vast and varied continent.
The Millennium Seed Bank Partnership (MSBP) has received the World Wide Biodiversity Conservation Award at the 16th BBVA Foundation Awards ceremony in Madrid, 30th November 2021. Each year the BBVA Foundation, the corporate social responsibility arm of the BBVA financial services group, hosts an award ceremony recognising those working for Biodiversity Conservation. Three awards are given, one for biodiversity conservation in Spain, one for knowledge dissemination and communication in biodiversity conservation in Spain, and one for worldwide biodiversity conservation.
It is this global category that the Millennium Seed Bank Partnership (MSBP) has won “for its extraordinary contribution to the preservation of the world’s plant biodiversity, through the creation of a seed bank holding 2.5 billion samples of plants from 190 countries”. The judges considered the MSBP to be an exemplary initiative which reflects how cooperation without borders can advance nature conservation worldwide and successfully address the central challenge of preserving biodiversity. They were particularly impressed by the MSBP's training capacity, a key element of the partnership which allows best practice, technology and ideas to be shared across the network.
Dr Elinor Breman, lead of the MSBP Initiative at Kew, travelled to Madrid to accept the award, with colleagues Dr Aisyah Faruk who coordinates our work across Europe, and Dr Tiziana Ulian, who works on diversity and livelihoods.
The award also provided 250,000 Euros to further the work of the MSBP. In the spirit in which the award was given, this funding is being used to continue our training programme and conserve critically endangered plants, bringing them back from the brink of extinction.
The funding will enable 10 people to undertake Technical Attachments at the Millennium Seed Bank and ~12 people to attend our flagship Seed Conservation Techniques course in 2022. The remaining funds will support a new four-year project to conserve 50 plant species designated as critically endangered (CR) by the global IUCN Red List.
Mind the Gap - What CR species are conserved?
The species to be conserved have been identified through an internal review of MSBP holdings of CR species, which identified CR species missing from collections as well as those whose collections could be improved (increase in the number of seeds from a population or the number of populations conserved). Ten countries will be involved in not only collecting these CR species, but drawing together the known information about them, developing propagation protocols and then feeding information and plants back into in situ conservation efforts.
The transfer of seed conservation knowledge through the Millennium Seed Bank Partnership (MSBP) over the last two decades has been reviewed by Way & Breman (2021). They show how the MSBP’s international training programme has successfully translated research knowledge into practical standards and guidelines. They also illustrate how the MSBP continues to develop decision-support tools such as the Seed Recalcitrance Predictor and the MSBP Data Warehouse and shares best practice in seed conservation through technical publications and partnership newsletters.
For 2022 we are hoping to run our Seed Conservation Techniques training course onsite at the Millennium Seed Bank (MSB) for the first time since 2018. The proposed dates include a week of online theory from September 26th- 30th, followed by practical hands-on sessions from October 9th - 22nd. Demand is likely to be high, so if you would like further information please contact MSBTraining@kew.org as soon as possible. The deadline for expressions of interest is April 30th.
In the December edition of Samara, we featured a news story on receiving seeds from the hibaku jumoku (survivor trees) from the Green Legacy Hiroshima Initiative. The trees survived the atomic bomb dropped on the city of Hiroshima, Japan, on August 6, 1945, at the end of the Second World War and are now grown around the world as a symbol of peace and hope.
Many of the species we received from the Hiroshima survivor trees need cold stratification treatment and are still in the fridge. Some however, needed no pre-treatments. They were sown straight away and put into our glasshouse at a cosy 22°C.
The Cinnamomum camphora (Camphor Tree) seeds were first to the mark, with all three germinating (the first germinating after 4 weeks). They will soon be potted into airpots and as they grow (and toughen up), acclimatised to cooler conditions. I look forward to the day when these survivors will be planted out in the gardens at Wakehurst!
Notes on the Begonias of Mt. Slamet, Central Java, Indonesia
Muhammad Efendi, Abidin Ibrahim, Dadang Sunandar, Intani Quarta Lailaty & Vandra Kurniawan (Cibodas Botanic Gardens, Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency)
Begonia is known as an ornamental plant by many plant enthusiasts. It has an interesting shape and colour to the leaves, and also has many varied colours of flowers (Tebitt, 2005). Several species of Begonia have other uses, for example B. comestibilis is used for food (Thomas et al., 2011), B. multangula as a spice, B. muricata (Hartutiningsih et al., 2009; Putri et al., 2019), B. medicinalis (Ardi et al., 2019) and B. baliensis (Hartutiningsih et al., 2018) were reported as potential medicines. These species contain antimicrobial compounds with the potential to be developed as medicinal raw materials.
Mount Slamet is known as habitat of native Begonia’s in Java. A total of five native Begonia have been reported in this area in a previous study (Efendi, 2019). A seed collecting expedition was conducted in the Mount Slamet forest area, Central Java, Indonesia, to increase the number of Begonia seed collections which are stored in the Cibodas Botanic Garden’s Seed Bank. A total of six species of native Begonia were found and collected within the South Slope area of Mount Slamet, namely B. multangula, B. longifolia, B. areolata, B. atricha, B. isoptera and B. muricata. A new location of Begonia isoptera was added to its range distribution from this expedition. Thus, as many as 40% of Java's native species of Begonias are now stored in Cibodas Botanic Garden’s Seed Bank and duplicated at the Seed Bank of the Center for Plant Conservation and Botanic Gardens. Unfortunately, there is a decreasing trend of the B. atricha population in Mt. Slamet caused by over exploitation for ornamental plants.
Siregar, H-M., Purwantoro, R.S., Praptiwi, P. & Agusta, A. (2018) Antibacterial potency of simple fractions of ethyl acetate extract of Begonia baliensis. Nusantara Bioscience. 10: 159-163. Available at: https://smujo.id/nb/article/view/2726
Putri, N.H.S., Nurdiwiyati, D., Lestari, S., et al. (2019)Aktivitas antibakteri ekstrak tangkai dan daunBegonia multangula Blume. terhadapPorphyromonas gingivalis. Jurnal Biologi Universitas Andalas. 7(1): 51-58.
Thomas, D.C., Ardi, W.H. & Hughes, M. (2011) Nine new species of Begonia (Begoniaceae) from South and West Sulawesi, Indonesia. Edinburgh Journal of Botany. 68(2): 225–255. DOI: https://doi.org/10.1017/S0960428611000072
Our collaboration with SANBI (The South African National Biodiversity Institute) was one of the first under the global Millennium Seed Bank Partnership (MSBP). In over 20 years of working together the MSBP/SANBI team have made more than 6,500 collections, covering more than 4,350 species.
We spoke to Victoria Wilman (Seed Conservation Programme Manager) to learn more about her team's incredible contribution and their hopes and plans.
What influenced you to work in seed conservation?
I used to spend hours hiking in the mountains and visiting South Africa’s National Parks. I loved plants and collected seeds wherever I could. When my grandmother passed away, she left me a small legacy, just enough to kick start my first year of studies. I wanted to do nature conservation, but knowing about the many rare and endangered plants, I decided to study horticulture, to be able to grow these plants to restore them back into the wild. After working in the Eastern Cape and completing my honours degree in Botany a few years later, I applied for a seed collecting position in Cape Town and started at SANBI in 2012. I have loved every minute of it!
How has the global pandemic influenced your work and personal life?
The pandemic stopped all of our fieldwork, and our teams collecting seeds around the country were grounded although some colleagues who live in remote areas continued to collect around their home villages. I have an amazing team, and despite all the Covid challenges, they continued to work extremely hard and remained dedicated to the MSBP project, with the result that we are not far behind our collecting targets.
How do you think SANBI has developed its commitment to Seed Conservation over the past 20 years?
SANBI's commitment to seed conservation is very strong, the seed room at Kirstenbosch National Botanical Gardens (NBG) has changed from a commercial operation to a Seed Conservation Unit servicing the whole of SANBI.
A Seed Conservation Programme is being developed which includes infrastructure for South Africa’s first wild plant seed bank at Kirstenbosch NBG, including dry rooms, a -20°C; freezer, germination and seed processing labs, all due to be completed in early 2023. This exciting project brings together a lifetime’s ambition for me.
The seed conservation programme is well integrated into SANBI’s other directives and many of SANBI’s permanent staff have come through the MSBP programme. Our seed collectors are stationed at four of SANBI’s Botanical Gardens and work together with horticulturists and curators. We work closely with the Threatened Species Programme and Custodians of Rare and Endangered Wildflowers (CREW) who manage volunteers monitoring threatened species, and we also train them in seed collecting for the project.
You have a strong Seed Conservation team at SANBI. Can you tell us a bit about the current team of researchers, their strengths and aspirations?
The team is comprised of myself, four seed collectors, one seed processing coordinator with support staff and an intern. We are finalizing two new projects at Kirstenbosch and Karoo Desert NBG which will add 3 more to the team.
Naomi Mdayi – Kirstenbosch NBG (Western Cape)
Sibahle Gumede – Kwelera NBG (Eastern Cape)
Fergy Nkadimeng – Pretoria NBG (Gauteng, Freestate, North West)
New Project - Karoo Desert NBG (Northern Cape), succulent poaching
New Project – Kirstenbosch NBG, Restoration Unit, succulent poaching
Seed Processing at Kirstenbosch NBG:
Thembeka Malwane – seed processing coordinator
Georgina Wilkinson – seed cleaning and packaging shipments
Deon Smith – Administration (requests for material and other Gardens work)
Siyabonga Magladla – seed cleaning
Patrick Kettledas – seed cleaning and collecting
Due to the vast and varied terrain of South Africa the scope of our work is immense. The seed collectors (all female) spend extended periods away and are always accompanied in the field for their security. They are dedicated and knowledgeable. Amongst their many talents they also train students and volunteers, as well as coordinate the field trips.
Sibahle Gumede, from Kwelera NBG in the Eastern Cape shared her thoughts:
My aspirations are to see a South Africa that no longer has plants which are threatened, to have independent volunteers to carry out the MSBP vision, and universities, landowners and environmental stakeholders taking ownership for seed conservation. And lastly to see satellite seed banks for each province, to be able to curate their endemic seeds.
Your team engages with volunteers across South Africa, what are the key factors that make these partnerships work and are there any challenges?
Most of our stakeholders, partners, and volunteers understand and have experience of the effects of not preserving indigenous species. Our partnership with them is symbiotic and mutual and we are extremely grateful for all the help we receive from volunteers who help find target species and even collect seeds. We occasionally have difficulties getting hold of landowners for site access and they may prohibit us from collecting, and we can sometimes be viewed as a threat that exploits vegetation to give away seeds to another country, but we work hard at spreading the conservation message and more often than not receive cooperation and enthusiastic assistance.
You alone have been involved with 500 of the collections made by the MSBP/SANBI team. Do you have any collection highlights?
One of my favourite experiences is collecting from Silvermine Nature Reserve after a huge fire in 2015, which many viewed as devastating. We knew it was just the beginning of another cycle in nature and we discovered many species which were previously not present and some that come up en masse after a fire, like the beautiful Aristea spiralis, Disa racemosa and Erica amoena.
Another great memory was collecting Euryops indecorus, a Critically Rare species known from only one site on cliff faces above Rooiels in the Western Cape. It took a whole day to get to and climb to the top of the mountain where this plant occurs and what a joyful and exciting feeling to find the species in flower and in seed, and to know that it is now secured in the seedbank.
We know that SANBI is pivotal in driving in situ species conservation and recovery, are there any key initiatives for which your seed collections will be used?
In partnership with MSBP, CREW, Kirstenbosch NBG and Stellenbosch Botanical Garden we are working on the Marasmodes undulata project. This species is classified as Critically Endangered and only occurs at a single locality. When last monitored, only three plants were present. The site has since burned, leaving no plants left in the wild. Seeds originally collected in 2005 were taken out of the seedbank and germinated at Kirstenbosch NBG and the Stellenbosch University BG. These plants are the basis for restoring this species, and these, and additional cuttings taken from them, will be planted into the wild this Autumn. It shows how important having ex situ collections can be.
How do you see the role SANBI plays in Southern Africa?
SANBI plays a critical role in the conservation arena, it leads and coordinates conservation research, and monitors and reports on the state of biodiversity in South Africa while also managing 10 Botanical Gardens that create awareness and showcase South Africa’s plants and provide education programs for learners. SANBI pilots wild plant seed conservation through our MSBP and seed conservation programme and highlights its importance through training programmes to many other organisations.
What do you see as the future challenges you are facing in your work?
Succulent poaching is a major problem, with plants exported for their ornamental value and the safety of my teams in the field is always a concern. Managing the new seed bank and developing a good seed science programme will be a new challenge, but a wonderful and exciting one.
You recently attended the remote Seed Conservation Techniques (SCT) course. How did you find it, and do you have any plans to further develop your current in-country training programme for staff and volunteers?
It was a great course; I really enjoyed it and learned so much. It was well organised with many resources. We are embarking on a staff training day every month, covering one aspect of the SCT training, and we will use the resources and videos from the course.
Detailed alternative text for map
A map of the provinces of South Africa showing the location of the different MSBP teams and the National Botanic Gardens (NBG) they’re based at, plus their respective collecting areas. The MSBP team based at Thohoyandou NBG in Limpopo cover collecting areas within the provinces of Limpopo and Mpumalanga. The MSBP team based at Pretoria NBG in Gauteng province cover collecting areas within the provinces of Gauteng, North West and Free State. The MSBP team based at Kwelera NBG cover collecting areas within the Eastern Cape and KwaZulu-Natal provinces. There are two MSBP teams within the Western Cape, one at the Karoo Desert NBG and one at Kirstenbosch NBG, these cover collecting areas within the Northern Cape and Western Cape provinces.
How do you perform a cut test on very small seeds, especially when in the field?
There are two elements to handling small seeds. One is to make sure they stay in place and don’t fly away, the other is to use the right tool for cutting.
We suggest the use of adhesive tape, by taking a strip of the tape and creating a loop (with the adhesive side facing outwards to fix the seeds), and then using small scissors to cut the seeds (either on the tape surface or by cutting through the tape with seed in place) (Fig. 1). Experienced collectors may also use their fingernail for cutting. It is always helpful to use a hand lens or microscope when cut testing very small seeds. Very tiny seeds, such as those of orchids, can’t be effectively cut tested.
Recent publications from across the MSBP:
Diantina, S., McGill, C., Millner, J., Nadarajan, J., Pritchard, H.W., Colville, L. & Clavijo McCormick, A. (2022) Seed viability and fatty acid profiles of five orchid species before and after ageing. Plant Biology. 24: 168-175. DOI: https://doi.org/10.1111/plb.13345
Faruk, A., Papikyan, A. & Nersesyan, A. (2021) Exploring effective conservation of charismatic flora: orchids in Armenia as a case study. Diversity. 13(12): 624. DOI: https://doi.org/10.3390/d13120624
Liu, D., Cai, J., He, H., Yang, S., Chater, C.C.C. & Yu, F. (2022) Anemochore seeds harbour distinct fungal and bacterial abundance, composition, and functional profiles. Journal of Fungi. 8(1):89. DOI: https://doi.org/10.3390/jof8010089
Kallow, S., Garcia Zuluaga, M., Fanega Sleziak, N., Nugraha, B., Mertens, A., Janssens, S.B., Gueco, L., Valle-Descalsota, M.L., Dang Vu, T., Toan Vu, D., Thi Li, L., Vadeloo, F., Dickie, J.B., Verboven, P., Swennen, R. & Panis, B. (2022) Drying banana seeds for ex situ conservation. Conservation Physiology. 10: coab099. DOI: https://doi.org/10.1093/conphys/coab099
Pence, V.C., Meyer, A., Linsky, J., Gratzfeld, J., Pritchard, H.W., Westwood, M. & Bruns, E.B. (2022) Defining exceptional species - A conceptual framework to expand and advance ex situ conservation of plant diversity beyone conventional seed banking. Biological Conservation. 266: 109440. DOI:https://doi.org/10.1016/j.biocon.2021.109440
Pence, V.C., Bruns, E.B., Meyer, A., Pritchard, H.W., Westwood, M., Linsky, J., Gratzfeld, J., Helm-Wallace, S., Liu, U., Rivers, M. & Beech, E. (2022) Gap analysis of exceptional species - Using a global list of exceptional plants to expand strategic ex situ conservation action beyond conventional seed banking. Biological Conservation. 26: 109439. DOI: https://doi.org/10.1016/j.biocon.2021.109439
Visscher, A.M., Boatfield, M., Klak, C., Yeo, M., Pearce, T.R., Wilman, V., Mdayi, N., Gumede, S. & Pritchard, H.W. (2022) Physiological seed dormancy of Ruschia imbricata and Ruschia uitenhagensis (Aizoaceae) is broken by dry heat and unaffected by seasonality. South African Journal of Botany. 147: 457-466. DOI: https://doi.org/10.1016/j.sajb.2022.02.007
Important notice regarding the Seed Information Database
A recent assessment of the Seed Information Database (SID) for compliance with UK legislation on website accessibility (Public Sector Bodies [Websites and Mobile Applications] Accessibility Regulations 2018; themselves aligned with the Web Content Accessibility Guidelines (WCAG) 2.1 at the AA standard) found the web interface deficient in several respects. Regrettably, due to RBG Kew’s need to prioritise its limited resources, we will be unable to remedy the faults; and the SID web interface will be taken down at the end of this financial year (March 2022).
However, we will continue to update SID's data content internally; and this will continue to be available to external users soon as flat files from RBG Kew's FTP (file transfer portal) site.
Please also note that for many years we have shared much of SID's seed functional trait data via the TRY Plant Trait Database, which facilitates bulk data downloads; and we intend to continue periodic updates of that content.
In the future, as far as possible, we plan to deliver species' seed trait information via Kew's 'Plants of the World Online' portal, which should be convenient for users browsing or searching for data on individual species. Moreover, Kew's current Science Strategy sets out (pg. 27) an ambition to collaboratively deliver a 'Global Seed Information Facility'.
Kew launches two new MSc courses
As part of Kew's ambitious commitment to training the next generation of biodiversity scientists, we're expanding our postgraduate training offer with the launch of two new MSc courses. The MSc programmes offer the opportunity to study at Kew, with unrivalled access to our scientific collections, laboratory facilities, partnerships and landscapes.
Students can now choose between three MSc programmes at Kew in partnership with Queen Mary University of London or Royal Holloway, University of London:
This issue we hear about field work activities in Australia, Mexico, Pakistan and South Africa; we interviewed Janet Terry who retired from the Millennium Seed Bank in July after more than 30 years and we outline the current plant health requirements for shipping plant material to the UK.
Pakistan is rated as ‘comparatively’ forest-poor by the FAO (Food and Agriculture Organisation of the United Nations), with only 0.05 ha of forest per capita compared with a global average of 1.0 ha in 2019 (FAO, 2002). In addition, the severe impacts of a changing climate, the high rate of population growth (1.95%) and the subsequent pressure on land, together with a high demand for forest products, is placing additional strains on the country’s forest cover.
In rural areas, wood is an important commodity for fuel and timber, which is driving forest clearance at an alarming rate (90% of households are reliant on wood for fuel). And additional pressures exist due to severe overgrazing and the conversion of forests for agriculture.
IUCN conservation assessments show that many trees used for their medicinal properties in Pakistan are threatened with extinction, such as the Critically Endangered Commiphora wightii (Mukul myrrh tree), while Taxus wallichiana (Himalayan yew) and Rhamnella gilgitica are classified as Endangered and Vulnerable respectively. Much research still needs to be carried out on other species to lift them from the status of Data Deficient, such as Cercis griffithii (Afghan rebud), Malus chitralensis (Chitral crab apple) and Prunus bokharienis.
A two-year project funded by the Garfield Weston Foundation in collaboration with the Pakistan Bio-resources Conservation Institute (BCI) is aiming to collect the seeds of at least 70 rare, threatened and over-exploited useful tree and shrub species in Pakistan. This project (part of the Global Tree Seed Bank Programme) follows on from the successful partnership between RBG Kew and BCI in the Medicinal and Aromatic Plants of Pakistan Project – MAPs (2019) and the Adapting Agriculture to Climate Change (Crop Wild Relative - CWR) project (2016-2018). Project funding has purchased essential equipment needed to increase the capacity of BCI staff to conserve seed collections, such as a seed aspirator, an incubator-drier, a freezer, and tree seed collecting equipment. Additional funding secured from the Alan Lennox Boyd Memorial Trust was used to purchase reference books and herbarium supplies.
Despite delays to the project due to COVID-19, the team in Pakistan led by Dr. Sadar Uddin Siddiqui (Chief Scientific Officer/Curator of the National Genebank of Pakistan and Director of the Bio-resources Conservation Institute) has been able to collect seeds and herbarium specimens from plants belonging to 18 families, 29 genera and 31 species. Collections in the 2020/2021 season were made across a variety of habitat types and include rarities in Pakistan, such as Wrightia arborea (Woolly dyeing rosebay) from which the root, bark and leaves can be used in traditional medicine, as well as Butea monosperma (Flame of the forest) and the climate change-resilient, but underutilized, Sideroxylon mascatense. This plant is a small, but viciously spiny shrub, that produces a sweet edible purple fruit (a bit like a plum), which is believed to have the potential to be brought more widely into cultivation.
FAO (2002) An overview of forest products statistics in South and Southeast Asia. Eds. Ma, Q. & Broadhead, J.S.. EC-FAO Partnership Programme.
The Succulent Karoo biome stretches along the west coast of South Africa, spanning an area of approximately 111,000 km2 between southern Namibia and the uplands of the Western Cape Province. It is home to 5000 vascular plant species, 40% of which are endemic. The world’s richest succulent flora occurs here. This diversity is remarkable for a region that receives 100 to 200 mm of rain annually (Mucina et al. 2006). In addition to this limited winter rainfall, the plants depend on the contributions of fog, dew and water vapour to meet their water requirements (Matimati et al. 2012).
In the past two years, an unprecedented upsurge in wild plant poaching from the Succulent Karoo and the adjacent Nama Karoo biomes has escalated the need for conservation in these regions. The partnership between RBG Kew’s Millennium Seed Bank (MSB) and the South African National Biodiversity Institute (SANBI) has achieved over 7500 seed collections of native South African species to date. However, the species of the Succulent Karoo are relatively under-collected due to the high endemism of this biome and the remote locations of populations, which make accessibility for seed collections difficult. Thus, there are still many hundreds of rare and threatened plant species yet to be secured by seed banking.
The recent poaching increase is the result of a new interest in caudiciform and dwarf succulent species on foreign markets. Since mid-2019 there has been an increase in the number of poacher arrests and confiscation of wild plant material, which is brought to SANBI for identification, expert statements, and curation. In 2019 SANBI received on average 400 confiscated plants per week, which rose dramatically in 2021, to over 1500 plants per week. The genus Conophytum (Aizoaceae) has been especially targeted by poachers, while other confiscated genera include Anacampseros (Anacampserotaceae), Lithops (Aizoaceae) and Portulacaria (Portulacaceae). Many of these succulent species are already threatened and in some cases are critical habitat species, meaning that they only occur in an area less than 10 km2. This is the case for 48 Conophytum species, which occur as single populations, often on one ridge of a single mountain. Rare populations such as these are easily driven to extinction through over harvesting and several Conophytum species may already be extinct as a direct result of recent poaching.
Poaching compounds the pressure on these species from climate change. Models based on the key climatic and geological variables that influence Conophytum distribution predict a severe contraction of suitable habitat for most of the genus, increasing their extinction risk (Young et al. 2016).
In efforts to save these species from extinction in the wild, MSB South Africa has joined with the Custodians of Rare and Endangered Wildflowers (CREW), a SANBI programme that surveys and documents plant taxa of conservation concern, providing data essential for updating the IUCN Red List. A new MSB Karoo biome extension project is also currently being developed to respond to this conservation crisis.
There is now a race to bank the seeds before populations are decimated by poaching. Earlier this year MSB South Africa joined CREW on a field trip to assess and bank seeds of the species currently under the most poaching pressure. It was incredible to see the effort that goes into succulent poaching. To find them, the team had to scramble up steep rocky mountainsides under the blazing sun in the dry expanse of the Northern Cape, our vehicles becoming specks in the distance. The dry remains of succulents that had succumbed to the severe drought in recent years were scattered across the landscape, a reminder of the delicate balance of this region and the losses that may be brought about by climate change.
Species of the genus Conophytum grow as single bodies (smaller than your thumb) or in small dense clusters that camouflage well with their rocky surroundings. They often grow tucked into rock crevices and (in some cases) are only present on impossible to reach cliff faces, which may indicate that the more accessible plants have already been poached. In two cases, poachers were at the sites only a few hours before us. It was a privilege to see these species in their natural habitats, but tragic to know that if the poaching continues, we might be some of the last. Yet, there is still hope for these species, in the seeds of 11 species collected on that trip and others that will soon be stored in the vaults of the Millennium Seed Bank, protecting these species for the future.
Matimati, I., Musil, C.F., Raitt, L. & February, E. (2012) Non rainfall moisture interception by dwarf succulents and their relative abundance in an inland arid South African ecosystem. Ecohydrology. 6: 818-825. https://doi.org/10.1002/eco.1304.
Mucina, L., Jürgens, N., Le Roux, A., Rutherford, M.C., Schmiedel, U., Esler, K., Powrie, L.W., Desmet, P.G. & Milton, S.J. (2006) Succulent Karoo Biome. In: Mucina, L., Rutherford, M.C. (Eds.) The Vegetation of South Africa, Lesotho and Swaziland. Strelitzia 19. South African National Biodiversity Institute, Pretoria. pp. 220-299.
Bridging the gap between the US Rocky Mountains and the Mexican Sierra Madre is a complex of 50 mountain ranges known as the Madrean ‘Sky islands’ Archipelago. With a diversity of habitats from desert shrubland to pine-oak woodlands, arranged across varied topography, the region is a priority for research and conservation, but botanical collections are under-represented from the Mexican territory (Deyo et al. 2013) and climate change is projected to erode and fragment the montane habitats (Yanahan & Moore 2018) before they have been fully studied.
Through the MSB Partnership, the Seed Bank of Facultad de Estudios Superiores Iztacala Universidad Autónoma de Mexico (FES-I UNAM) and RBG Kew have cooperated since August 2018 with the Herbarium of the University of Sonora (USON), in the important effort to conserve the Sonoran flora through seed banking. The aim was to diversify the collections of the seed banks involved and support their research studies, by collecting at least 150 accessions of native species of interest. Using USON in Hermosillo as the main base, field work was concentrated in 17 municipalities of Sonora State, as illustrated on the map in Fig. 1.
During the 2018 and 2019 collection season, teams comprising staff from Kew, UNAM, and USON worked together in the field. The work was intense, the months of June, August and October of 2018 were the best for seed collecting. To maintain safety in these border regions the team were careful to seek cooperation with landowners and other biologists in the field. Although the 2020 health emergency forced many activities to stop, fortunately a team of local collectors located in Guaymas, led by Pablo Carrillo (Fig. 2), worked very hard despite the restrictions and managed to collect many species of interest.
Through the efforts of the teams, we have just finished the project with even better than expected results. In total, 244 accessions representing 44 botanical families, 147 genera and 194 species were collected and conserved, of which 49 are endemic species. Three species are included in some category of risk in accordance with the Official Mexican Standard NOM-059 SEMARNAT 2010, 14 are in Appendix II of CITES and 17 are in the Least Concern (LC) category of the IUCN.
All the species collected have biological, ecological, or economic importance, however the following species are especially emblematic:
The Guayacán, Guaiacum coulterii A. Gray, a native tree of the thorny scrub of Sonora, has medicinal properties and its wood is useful for construction as well as being very beautiful as an ornamental plant and a source of food for different species of pollinators. It is classified as threatened in Mexico and vulnerable internationally (Fig. 3 & 4).
The Cabeza de viejo (‘Old Man's Head’), Lophocereus schottii (Engelm.) Britton & Rose, is a columnar cactus which has medicinal properties, found in Sonora’s thorny scrub and dunes. It is classified as subject to special protection in Mexico.
The Saguaro, Carnegiea gigantea (Engelm.) Britton & Rose, is perhaps one of the most appreciated species for its beauty in Arizona and Sonora. It has threatened status in Mexico.
A Nopal, an Opuntia spp., collected in the Sierra de Mazatán, is probably a new species for science that is being described by specialists.
In conclusion, the state of Sonora is, without a doubt, a place rich in plant diversity that should continue to be studied and conserved. Much remains to be discovered, collected and conserved in the most remote and inhospitable areas that are unfortunately not very easy to access.
Thanks to this project, valuable information has been created on the flora of the sky islands within Sonora, and knowledge has been developed on how security can be maintained within the area, also on the bi-national distribution of species that are threatened in the United States and little known from Mexico. We acknowledge generous support of John and Catherine Emberson to the Kew Foundation to enable this project to happen.
Yanahan, A.D. & Moore, W. (2018) Impacts of 21st-century climate change on montane habitat in the Madrean Sky Island Archipelago. Diversity and Distributions. 25: 1625-1638. https://doi.org/10.1111/ddi.12965
In the lab we’ve also isolated fungus for a number of threatened orchid species lately for some symbiotic in-vitro propagation work later this year. We’ve been trialling some methods for some difficult species so we were recently delighted (and relieved) to see some success for black-beaked duck-orchid (Caleana disjuncta). We’re doing further research on the Copper beard-orchid (Calochilus cupreus) at the moment for ‘Green Adelaide’ so we’re hoping for similar success before we take off into the wilds again. The world count got up to nearly 40 plants last year so we’re hoping to augment this with propagated plants…and find more populations.
On the 27th August, we put on a display at Adelaide Botanic Garden of some of the endangered orchids (12+ species) we’re currently propagating for the ‘Back from the Brink’ project. This was a good opportunity for landholders, volunteers, partners and sponsors to see some of these interesting orchids in flower.
Kolkhuri jonjoli, Staphylea colchica Steven
Ian Willey (RBG Kew)
Staphylea colchica is native to the Caucasus region where it is known commonly as kolkhuri jonjoli (კოლხური ჯონჯოლი Georg.), Colchis Bladdernut, Caucasian Bladdernut, Jonjol or the Jonjoli tree. Staphylea colchica is a deciduous shrub or small tree growing up to 4 metres in height with three or five ovate leaflets with finely toothed margins. In spring its small, white, fragrant bell-shaped flowers are borne on racemes and develop into greenish-white bladder-like papery fruits containing orange seeds (hence its name “bladdernut tree” in English). The flowering shoots with buds of ‘Jonjoli trees’ are edible and are pickled by fermenting in brine to produce Jonjoli, a revered delicacy in the Caucasus, and in Georgia are a common feature of the traditional feast “Supra”.
People from across the Caucasus region will have eaten Jonjoli, and an export market exists in countries with a significant Caucasian diaspora. S. colchica’s cultural value is both a blessing and a curse. Jonjoli occupies a special place in the region’s cultural identity with the tree recognisable and cherished by many. However, wild populations are threatened by the over collecting of reproductive parts for culinary purposes. Harvesting the flowering shoots and buds limits the plants’ ability to reproduce from seed, further decreasing the viability of source populations. Additionally, S. colchica distribution is severely fragmented across its native range, and the number of individual plants per population is low. A preliminary assessment in 2005 reports that distribution in the Caucasus has reduced by 50%, but a full global IUCN Red List assessment remains incomplete, and more field data is needed to establish a reliable baseline. Taxonomic studies are needed to validate species status and more research is needed to understand threats to the species and the true rate of decline.
Two ex situ seed collections of S. colchica are banked in Georgia and duplicated to the MSB. These collections are a key component of efforts to protect this species and seeds can be made available for use dependant on prior consent from partner institutions. However, S. colchica requires greater protection in situ. Overharvesting and land-use change are very real threats but by promoting sustainable harvesting and engaging communities, as was done in the Darwin Initiative-funded Enhancing rural Caucasian livelihoods through fruit and nut conservation, this culturally significant plant can be protected.
More information on other species conserved through the project can be found in the project booklet (pdf).
A fond farewell to Janet Terry
Circa 1984, when Janet began a Saturday job working in the restaurant at Wakehurst, she could not have envisaged the 3-decade long career which would follow, when she would go on to travel the world and meet, train, and collect with “so many of our wonderful partners” and “see so many strange and unique seeds and know we have them safely stored”.
When an opportunity arose with the International Board for Plant Genetic Resources (IBPGR, now The Crop Trust) which was running a seed handling unit at Wakehurst, Janet applied for the job as “it sounded interesting!”. Teams went out in the field (predominantly Africa) to collect cultivars and specimens of all the major crops. These got sent back to Janet, who had to sort and distribute them to the crop gene banks which were working on them. In 1990 a seed processing job came up with Kew and Janet moved to work for Kew. Work started on setting standards for collecting and storing seeds. As Janet says, these standards remain largely unchanged today – “keep it dry, keep it cool and it will live longer”.
We asked Janet to reflect on her incredible journey:
What will you miss most about working at the MSB? The people – definitely! Especially the contact with counterparts in equivalent positions around the world, who we can share ideas and experiences with.
What's the favourite species you have handled? The grapple - Harpagophytum. We collected them in Botswana. They were quite challenging to collect and even more so to clean!
What's your first memory of working here? That the Seedbank was in the Mansion – Lady Price’s bedroom was the lab and Sir Henry Price’s bathroom was the x-ray room. The stark contrast of working in an Elizabethan mansion whilst concentrating on cutting edge work! There was a large computer which took up a whole room in the Orchard building. In those early days the field data sheets were loaded onto this huge computer, so from the outset the data has been stored digitally.
What advice would you give to somebody about how to clean a collection? Slowly and carefully!
How do you see seed processing changing in the future? Funding for ‘business as usual’ processing is difficult. People love to fund new and exciting projects, but we need to be able to sustain the day-to-day processing, otherwise we may end up without processing roles and just dry and store seed at -20°C. Without viability testing, we cannot distribute the seed. This highly skilled work by practical applied scientists needs to be supported. I wish their skills were valued more highly. It’s also learning from others – the partnership, collaboration and knowledge that is the MSB’s strength.
If you hadn't worked here, what else might you have liked to do? When I worked for IBPGR, I went to a conference at their headquarters in Rome and a network contact from Indonesia offered me a job working with Orangutans. I was very tempted - things could have been very different!
What are you planning to do in your retirement? Nothing at first - that’s the point! Then I’ll probably get a part time job, working outdoors.
If you could be one plant, what would it be and why? A Welwitschia, as it’s slow growing and they live for an awfully long time. I would sit and watch the world go by!
Somehow, we can’t imagine Janet letting the grass grow under her feet for very long! Thank you to Janet for sharing her thoughts and memories with us.
What are the new requirements for sending plant material to the MSB?
Due to the UK leaving the European Union, there have been changes to Plant Health rules for shipping plant material to the UK.
All seed collections coming into the MSB from outside the UK now need to come with a phytosanitary certificate issued by the relevant authority in the exporting country. A phytosanitary certificate is an official document declaring that plants and plant material are free from pests and diseases. If seed collections arrive with this documentation, then none of the collections are classed as quarantine and the seeds and plant material will be processed as normal. This applies to wild collected and commercial seeds and plant material. The processing and long-term storage of the collections will be no different.
If a phytosanitary certificate cannot be obtained, then material will need to come in with a Letter of Authority (LoA) from the UK Plant Health Service, which can be obtained from the MSBP Conservation Coordinator for your region. However, any material coming in under the LoA will be treated as 'quarantine' upon arrival and restrictions will be placed on the future use of these collections.
As usual, before seed collections are dispatched to the MSB, species lists should be sent to the Seed Collections Manager who will check the list for any prohibited species and CITES-listed species (the Convention on International Trade in Endangered Species of Wild Fauna and Flora).
Copies of all documents should be attached to the outside and also put inside the shipping box. Please also send the airwaybill (AWB) number and copies of the documents to the Seed Collections Manager, so the consignment can be tracked.
Ibrahimova, A.G., Gasimova, K.H., Breman, E., Faruk, A., Willey, I. & Ali-zade, V.M. (2020) Morphological characteristics of seed of the endangered species of Diospyros lotus (Ebenaceae) in Azerbaijan. Plant & Fungal Research. 3: 52-60. http://dx.doi.org/10.29228/plantfungalres.77
Margreiter, V., Walde, J. & Erschbamer, B. (2021) Competition-free gaps are essential for the germination and recruitment of alpine species along an elevation gradient in the European Alps. Alpine Botany. https://doi.org/10.1007/s00035-021-00264-9
Müller, J.V., Cockel, C.P., Gianella, M. & Guzzon, F. (2021) Treasuring crop wild relative diversity: analysis of success from the seed collecting phase of the 'Adapting Agriculture to Climate Change' project. Genetic Resources and Crop Evolution. https://doi.org/10.1007/s10722-021-01229-x
Newton, R.J., Mackenzie, B.D.E., Lamont, B.B., Gomez-Barreiro, P., Cowling, R.M. & He, T. (2021) Fire-mediated germination syndromes in Leucadendron (Proteaceae) and their functional correlates. Oecologia. 196: 589-604. https://doi.org/10.1007/s00442-021-04947-2
Updates to the field data form
We are currently in the process of updating the field data form to improve its compatibility with the data required for IUCN threat assessments. We expect the update to be ready before the end of December.